TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis

Lei Gao, Dantong Li, Ke Ma, Wenjuan Zhang, Tao Xu, Cong Fu, Changbin Jing, Xiaoe Jia, Shuang Wu, Xin Sun, Mei Dong, Min Deng, Yi Chen, Wenge Zhu, Jinrong Peng, Fengyi Wan, Yi Zhou, Leonard I. Zon, Weijun Pan

Research output: Contribution to journalArticle

Abstract

In vertebrate definitive hematopoiesis, nascent hematopoietic stem/progenitor cells (HSPCs) migrate to and reside in proliferative hematopoietic microenvironment for transitory expansion. In this process, well-established DNA damage response pathways are vital to resolve the replication stress, which is deleterious for genome stability and cell survival. However, the detailed mechanism on the response and repair of the replication stress-induced DNA damage during hematopoietic progenitor expansion remains elusive. Here we report that a novel zebrafish mutantcas003 with nonsense mutation in topbp1 gene encoding topoisomerase II β binding protein 1 (TopBP1) exhibits severe definitive hematopoiesis failure. Homozygous topbp1cas003 mutants manifest reduced number of HSPCs during definitive hematopoietic cell expansion, without affecting the formation and migration of HSPCs. Moreover, HSPCs in the caudal hematopoietic tissue (an equivalent of the fetal liver in mammals) in topbp1cas003 mutant embryos are more sensitive to hydroxyurea (HU) treatment. Mechanistically, subcellular mislocalization of TopBP1cas003 protein results in ATR/Chk1 activation failure and DNA damage accumulation in HSPCs, and eventually induces the p53-dependent apoptosis of HSPCs. Collectively, this study demonstrates a novel and vital role of TopBP1 in the maintenance of HSPCs genome integrity and survival during hematopoietic progenitor expansion.

Original languageEnglish (US)
Article numbere1005346
JournalPLoS Genetics
Volume11
Issue number7
DOIs
StatePublished - Jul 1 2015

Fingerprint

DNA topoisomerase (ATP-hydrolysing)
Type II DNA Topoisomerase
hematopoiesis
Hematopoiesis
Zebrafish
Hematopoietic Stem Cells
Danio rerio
cell viability
stem cells
binding proteins
Cell Survival
Carrier Proteins
stem
protein
DNA
damage
genome
DNA damage
DNA Damage
apoptosis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. / Gao, Lei; Li, Dantong; Ma, Ke; Zhang, Wenjuan; Xu, Tao; Fu, Cong; Jing, Changbin; Jia, Xiaoe; Wu, Shuang; Sun, Xin; Dong, Mei; Deng, Min; Chen, Yi; Zhu, Wenge; Peng, Jinrong; Wan, Fengyi; Zhou, Yi; Zon, Leonard I.; Pan, Weijun.

In: PLoS Genetics, Vol. 11, No. 7, e1005346, 01.07.2015.

Research output: Contribution to journalArticle

Gao, L, Li, D, Ma, K, Zhang, W, Xu, T, Fu, C, Jing, C, Jia, X, Wu, S, Sun, X, Dong, M, Deng, M, Chen, Y, Zhu, W, Peng, J, Wan, F, Zhou, Y, Zon, LI & Pan, W 2015, 'TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis', PLoS Genetics, vol. 11, no. 7, e1005346. https://doi.org/10.1371/journal.pgen.1005346
Gao, Lei ; Li, Dantong ; Ma, Ke ; Zhang, Wenjuan ; Xu, Tao ; Fu, Cong ; Jing, Changbin ; Jia, Xiaoe ; Wu, Shuang ; Sun, Xin ; Dong, Mei ; Deng, Min ; Chen, Yi ; Zhu, Wenge ; Peng, Jinrong ; Wan, Fengyi ; Zhou, Yi ; Zon, Leonard I. ; Pan, Weijun. / TopBP1 Governs Hematopoietic Stem/Progenitor Cells Survival in Zebrafish Definitive Hematopoiesis. In: PLoS Genetics. 2015 ; Vol. 11, No. 7.
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