Tonic β-sympathetic activity in the lung periphery in anesthetized dogs

G. G. Weinmann, Ernst W Spannhake, B. Bromberger-Barnea, H. A. Menkes

Research output: Contribution to journalArticle

Abstract

The present study was undertaken to determine whether β-adrenoceptors could be physiologically detected in the lung periphery and whether they were under tonic stimulation in the resting state in anesthetized dogs. A fiberoptic bronchoscope was wedged in a sublobar segment of lung in anesthetized male mongrel dogs for measurement of resistance through the collateral system (Rcs). β-Agents were delivered locally as aerosols through the bronchoscope, and the response was evaluated by changes in Rcs. Distilled water alone produced a mean increase of 8.5 ± 2.43% (SE) in Rcs at 2 min in six dogs, whereas dl-isoproterenol produced a mean decrease of 8.9 ± 2.10% (P <0.03), thus demonstrating the presence of submaximally stimulated β-receptors. To test whether the β-receptors were under tonic stimulation, we compared the effect of aerosolized d- and dl-propranolol in 5 dogs. d-Propranolol that lacks significant β-blocking activity and dl-propranolol both produced large transient increases in Rcs. However, with d-propranolol, Rcs had returned to base line at 15 min, whereas with dl-propranolol Rcs remained elevated at a mean of 20% above base line for >2 h (P <0.01). Local timolol aerosol also produced a sustained increase in Rcs. After pretreatment with reserpine or after bilateral adrenalectomy, both d- and dl-propranolol still produced large transient increases in Rcs, but dl-propranolol no longer produced a sustained increase. Neither isoproterenol nor atropine affected Rcs in the presence of dl-propranolol, nor did pretreatment with atropine affect the response of Rcs to dl-propranolol. We conclude that β-adrenoceptors in the lung periphery are under tonic stimulation in the anesthetized dog from adrenally donated circulating catecholamines, and that the increase in Rcs after propranolol cannot be attributed to cholinergic tone.

Original languageEnglish (US)
Pages (from-to)979-984
Number of pages6
JournalJournal of Applied Physiology
Volume59
Issue number3
StatePublished - 1985

Fingerprint

Propranolol
Dogs
Lung
Bronchoscopes
Aerosols
Atropine
Isoproterenol
Adrenergic Receptors
Timolol
Reserpine
Adrenalectomy
Cholinergic Agents
Catecholamines
Water

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Weinmann, G. G., Spannhake, E. W., Bromberger-Barnea, B., & Menkes, H. A. (1985). Tonic β-sympathetic activity in the lung periphery in anesthetized dogs. Journal of Applied Physiology, 59(3), 979-984.

Tonic β-sympathetic activity in the lung periphery in anesthetized dogs. / Weinmann, G. G.; Spannhake, Ernst W; Bromberger-Barnea, B.; Menkes, H. A.

In: Journal of Applied Physiology, Vol. 59, No. 3, 1985, p. 979-984.

Research output: Contribution to journalArticle

Weinmann, GG, Spannhake, EW, Bromberger-Barnea, B & Menkes, HA 1985, 'Tonic β-sympathetic activity in the lung periphery in anesthetized dogs', Journal of Applied Physiology, vol. 59, no. 3, pp. 979-984.
Weinmann, G. G. ; Spannhake, Ernst W ; Bromberger-Barnea, B. ; Menkes, H. A. / Tonic β-sympathetic activity in the lung periphery in anesthetized dogs. In: Journal of Applied Physiology. 1985 ; Vol. 59, No. 3. pp. 979-984.
@article{2a667b13c81c4676baa718d85171b643,
title = "Tonic β-sympathetic activity in the lung periphery in anesthetized dogs",
abstract = "The present study was undertaken to determine whether β-adrenoceptors could be physiologically detected in the lung periphery and whether they were under tonic stimulation in the resting state in anesthetized dogs. A fiberoptic bronchoscope was wedged in a sublobar segment of lung in anesthetized male mongrel dogs for measurement of resistance through the collateral system (Rcs). β-Agents were delivered locally as aerosols through the bronchoscope, and the response was evaluated by changes in Rcs. Distilled water alone produced a mean increase of 8.5 ± 2.43{\%} (SE) in Rcs at 2 min in six dogs, whereas dl-isoproterenol produced a mean decrease of 8.9 ± 2.10{\%} (P <0.03), thus demonstrating the presence of submaximally stimulated β-receptors. To test whether the β-receptors were under tonic stimulation, we compared the effect of aerosolized d- and dl-propranolol in 5 dogs. d-Propranolol that lacks significant β-blocking activity and dl-propranolol both produced large transient increases in Rcs. However, with d-propranolol, Rcs had returned to base line at 15 min, whereas with dl-propranolol Rcs remained elevated at a mean of 20{\%} above base line for >2 h (P <0.01). Local timolol aerosol also produced a sustained increase in Rcs. After pretreatment with reserpine or after bilateral adrenalectomy, both d- and dl-propranolol still produced large transient increases in Rcs, but dl-propranolol no longer produced a sustained increase. Neither isoproterenol nor atropine affected Rcs in the presence of dl-propranolol, nor did pretreatment with atropine affect the response of Rcs to dl-propranolol. We conclude that β-adrenoceptors in the lung periphery are under tonic stimulation in the anesthetized dog from adrenally donated circulating catecholamines, and that the increase in Rcs after propranolol cannot be attributed to cholinergic tone.",
author = "Weinmann, {G. G.} and Spannhake, {Ernst W} and B. Bromberger-Barnea and Menkes, {H. A.}",
year = "1985",
language = "English (US)",
volume = "59",
pages = "979--984",
journal = "Journal of Applied Physiology",
issn = "0161-7567",
publisher = "American Physiological Society",
number = "3",

}

TY - JOUR

T1 - Tonic β-sympathetic activity in the lung periphery in anesthetized dogs

AU - Weinmann, G. G.

AU - Spannhake, Ernst W

AU - Bromberger-Barnea, B.

AU - Menkes, H. A.

PY - 1985

Y1 - 1985

N2 - The present study was undertaken to determine whether β-adrenoceptors could be physiologically detected in the lung periphery and whether they were under tonic stimulation in the resting state in anesthetized dogs. A fiberoptic bronchoscope was wedged in a sublobar segment of lung in anesthetized male mongrel dogs for measurement of resistance through the collateral system (Rcs). β-Agents were delivered locally as aerosols through the bronchoscope, and the response was evaluated by changes in Rcs. Distilled water alone produced a mean increase of 8.5 ± 2.43% (SE) in Rcs at 2 min in six dogs, whereas dl-isoproterenol produced a mean decrease of 8.9 ± 2.10% (P <0.03), thus demonstrating the presence of submaximally stimulated β-receptors. To test whether the β-receptors were under tonic stimulation, we compared the effect of aerosolized d- and dl-propranolol in 5 dogs. d-Propranolol that lacks significant β-blocking activity and dl-propranolol both produced large transient increases in Rcs. However, with d-propranolol, Rcs had returned to base line at 15 min, whereas with dl-propranolol Rcs remained elevated at a mean of 20% above base line for >2 h (P <0.01). Local timolol aerosol also produced a sustained increase in Rcs. After pretreatment with reserpine or after bilateral adrenalectomy, both d- and dl-propranolol still produced large transient increases in Rcs, but dl-propranolol no longer produced a sustained increase. Neither isoproterenol nor atropine affected Rcs in the presence of dl-propranolol, nor did pretreatment with atropine affect the response of Rcs to dl-propranolol. We conclude that β-adrenoceptors in the lung periphery are under tonic stimulation in the anesthetized dog from adrenally donated circulating catecholamines, and that the increase in Rcs after propranolol cannot be attributed to cholinergic tone.

AB - The present study was undertaken to determine whether β-adrenoceptors could be physiologically detected in the lung periphery and whether they were under tonic stimulation in the resting state in anesthetized dogs. A fiberoptic bronchoscope was wedged in a sublobar segment of lung in anesthetized male mongrel dogs for measurement of resistance through the collateral system (Rcs). β-Agents were delivered locally as aerosols through the bronchoscope, and the response was evaluated by changes in Rcs. Distilled water alone produced a mean increase of 8.5 ± 2.43% (SE) in Rcs at 2 min in six dogs, whereas dl-isoproterenol produced a mean decrease of 8.9 ± 2.10% (P <0.03), thus demonstrating the presence of submaximally stimulated β-receptors. To test whether the β-receptors were under tonic stimulation, we compared the effect of aerosolized d- and dl-propranolol in 5 dogs. d-Propranolol that lacks significant β-blocking activity and dl-propranolol both produced large transient increases in Rcs. However, with d-propranolol, Rcs had returned to base line at 15 min, whereas with dl-propranolol Rcs remained elevated at a mean of 20% above base line for >2 h (P <0.01). Local timolol aerosol also produced a sustained increase in Rcs. After pretreatment with reserpine or after bilateral adrenalectomy, both d- and dl-propranolol still produced large transient increases in Rcs, but dl-propranolol no longer produced a sustained increase. Neither isoproterenol nor atropine affected Rcs in the presence of dl-propranolol, nor did pretreatment with atropine affect the response of Rcs to dl-propranolol. We conclude that β-adrenoceptors in the lung periphery are under tonic stimulation in the anesthetized dog from adrenally donated circulating catecholamines, and that the increase in Rcs after propranolol cannot be attributed to cholinergic tone.

UR - http://www.scopus.com/inward/record.url?scp=0022410120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022410120&partnerID=8YFLogxK

M3 - Article

C2 - 2997106

AN - SCOPUS:0022410120

VL - 59

SP - 979

EP - 984

JO - Journal of Applied Physiology

JF - Journal of Applied Physiology

SN - 0161-7567

IS - 3

ER -