Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import

Koji Yamano, Yoh Ichi Yatsukawa, Masatoshi Esaki, Alyson E. Aiken Hobbs, Robert E. Jensen, Toshiya Endo

Research output: Contribution to journalArticle

Abstract

Precise targeting of mitochondrial precursor proteins to mitochondria requires receptor functions of Tom20, Tom22, and Tom70 on the mitochondrial surface. Tom20 is a major import receptor that recognizes preferentially mitochondrial presequences, and Tom70 is a specialized receptor that recognizes presequence-less inner membrane proteins. The cytosolic domain of Tom22 appears to function as a receptor in cooperation with Tom20, but how its substrate specificity differs from that of Tom20 remains unclear. To reveal possible differences in substrate specificities between Tom20 and Tom22, if any, we deleted the receptor domain of Tom20 or Tom22 in mitochondria in vitro by introducing cleavage sites for a tobacco etch virus protease between the receptor domains and transmembrane segments of Tom20 and Tom22. Then mitochondria without the receptor domain of Tom20 or Tom22 were analyzed for their abilities to import various mitochondrial precursor proteins targeted to different mitochondrial subcompartments in vitro. The effects of deletion of the receptor domains on the import of different mitochondrial proteins for different import pathways were quite similar between Tom20 and Tom22. Therefore Tom20 and Tom22 are apparently involved in the same step or sequential steps along the same pathway of targeting signal recognition in import.

Original languageEnglish (US)
Pages (from-to)3799-3807
Number of pages9
JournalJournal of Biological Chemistry
Volume283
Issue number7
DOIs
StatePublished - Feb 15 2008

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Mitochondria
Mitochondrial Proteins
Signal Transduction
Protein Precursors
Substrate Specificity
Substrates
Membrane Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Yamano, K., Yatsukawa, Y. I., Esaki, M., Aiken Hobbs, A. E., Jensen, R. E., & Endo, T. (2008). Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. Journal of Biological Chemistry, 283(7), 3799-3807. https://doi.org/10.1074/jbc.M708339200

Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. / Yamano, Koji; Yatsukawa, Yoh Ichi; Esaki, Masatoshi; Aiken Hobbs, Alyson E.; Jensen, Robert E.; Endo, Toshiya.

In: Journal of Biological Chemistry, Vol. 283, No. 7, 15.02.2008, p. 3799-3807.

Research output: Contribution to journalArticle

Yamano, K, Yatsukawa, YI, Esaki, M, Aiken Hobbs, AE, Jensen, RE & Endo, T 2008, 'Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import', Journal of Biological Chemistry, vol. 283, no. 7, pp. 3799-3807. https://doi.org/10.1074/jbc.M708339200
Yamano, Koji ; Yatsukawa, Yoh Ichi ; Esaki, Masatoshi ; Aiken Hobbs, Alyson E. ; Jensen, Robert E. ; Endo, Toshiya. / Tom20 and Tom22 share the common signal recognition pathway in mitochondrial protein import. In: Journal of Biological Chemistry. 2008 ; Vol. 283, No. 7. pp. 3799-3807.
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