TY - JOUR
T1 - Toll-like receptor 3 ligand polyinosinic:polycytidylic acid enhances autoimmune disease in a retinal autoimmunity model
AU - Ren, Xiangrong
AU - Zhou, Hongyan
AU - Li, Bing
AU - Su, Shao Bo
N1 - Funding Information:
This project was partially supported by the grants from the National Natural Science Foundation of China ( 81072483 ), the Science and Technology Planning Project of Guangdong Province, China ( 2009B030801170 ) and the Fundamental Research Funds of State Key Lab ( CX-01 ).
PY - 2011/6
Y1 - 2011/6
N2 - Viral components can trigger autoimmunity, but the involved mechanisms remain to be elucidated. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA (dsRNA) and appears to play an important role in this context. Our previous studies showed that signaling of TLR2, TLR3, TLR4 and TLR9 is highly redundant in the adjuvant effect needed to induce experimental autoimmune uveitis (EAU), an animal model of human autoimmune eye disease. In this study, we analyzed the effects of systemic delivery of polyinosinic: polycytidylic acid (poly(I:C)), a mimic of viral dsRNA, in the induction of EAU. We found that TLR3 agonist poly(I:C) enhanced EAU scores, DTH responses and Ag-specific T cell proliferation. In addition, Ag-specific Interleukin 17 (IL-17) and interferon gamma (IFN-γ) production by draining lymph node cells was markedly increased in the poly(I:C)-treated group. Our results suggest that activation of innate immune system mediated by TLR3 signaling pathway is of importance in the pathogenesis of virus-induced autoimmune diseases.
AB - Viral components can trigger autoimmunity, but the involved mechanisms remain to be elucidated. Toll-like receptor 3 (TLR3) recognizes viral double-stranded RNA (dsRNA) and appears to play an important role in this context. Our previous studies showed that signaling of TLR2, TLR3, TLR4 and TLR9 is highly redundant in the adjuvant effect needed to induce experimental autoimmune uveitis (EAU), an animal model of human autoimmune eye disease. In this study, we analyzed the effects of systemic delivery of polyinosinic: polycytidylic acid (poly(I:C)), a mimic of viral dsRNA, in the induction of EAU. We found that TLR3 agonist poly(I:C) enhanced EAU scores, DTH responses and Ag-specific T cell proliferation. In addition, Ag-specific Interleukin 17 (IL-17) and interferon gamma (IFN-γ) production by draining lymph node cells was markedly increased in the poly(I:C)-treated group. Our results suggest that activation of innate immune system mediated by TLR3 signaling pathway is of importance in the pathogenesis of virus-induced autoimmune diseases.
KW - Autoimmune disease
KW - Interleukin 17
KW - Polyinosinic:polycytidylic acid
KW - Toll-like receptor 3
KW - Uveitis
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U2 - 10.1016/j.intimp.2011.01.019
DO - 10.1016/j.intimp.2011.01.019
M3 - Article
C2 - 21296697
AN - SCOPUS:79955870871
SN - 1567-5769
VL - 11
SP - 769
EP - 773
JO - International immunopharmacology
JF - International immunopharmacology
IS - 6
ER -