Tolerogen-mediated suppression of the immune response. Suppressor cells as passive transfer agents

R. Z. Dintzis, M. H. Middleton, Howard M Dintzis

Research output: Contribution to journalArticle

Abstract

The anti-dinitro-phenyl (Dnp) IgM antibody response in mice was inhibited by administration of either a non-immunogenic form of Dnp-polyacrylamide (Dnp-Pa) or an excess amount of an immunogenic form of Dnp-Pa. Spleen cells, alive or heat-killed, from mice tolerized in vivo by either method, inhibited the anti-Dnp response of naive spleen cells co-cultured in vitro with antigen. Conversely, donor cells tolerized in vivo by a high dose of immunogenic Dnp-Pa, when titrated into a naive cell culture which contained no antigen, produced a stimulatory dose-response curve. Both the dose-dependent inhibition and stimulation correlated strongly with the amount of 125I-labelled Dnp-Pa carried over by tolerized spleen cells into the naive cell culture system. Because the doses and experimental procedures used were comparable to those commonly used for suppressor cell generation and assay, it is suggested that antigen-specific suppressor cells may produce their effects through passive transfer of antigen and/or tolerogen.

Original languageEnglish (US)
Pages (from-to)747-757
Number of pages11
JournalScandinavian Journal of Immunology
Volume28
Issue number6
StatePublished - 1988

Fingerprint

Antigens
Spleen
Cell Culture Techniques
Antibody Formation
Immunoglobulin M
Cultured Cells
Hot Temperature
Tolerogen
polyacrylamide
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Tolerogen-mediated suppression of the immune response. Suppressor cells as passive transfer agents. / Dintzis, R. Z.; Middleton, M. H.; Dintzis, Howard M.

In: Scandinavian Journal of Immunology, Vol. 28, No. 6, 1988, p. 747-757.

Research output: Contribution to journalArticle

@article{e7716f0869a9456d9709d2990d15155d,
title = "Tolerogen-mediated suppression of the immune response. Suppressor cells as passive transfer agents",
abstract = "The anti-dinitro-phenyl (Dnp) IgM antibody response in mice was inhibited by administration of either a non-immunogenic form of Dnp-polyacrylamide (Dnp-Pa) or an excess amount of an immunogenic form of Dnp-Pa. Spleen cells, alive or heat-killed, from mice tolerized in vivo by either method, inhibited the anti-Dnp response of naive spleen cells co-cultured in vitro with antigen. Conversely, donor cells tolerized in vivo by a high dose of immunogenic Dnp-Pa, when titrated into a naive cell culture which contained no antigen, produced a stimulatory dose-response curve. Both the dose-dependent inhibition and stimulation correlated strongly with the amount of 125I-labelled Dnp-Pa carried over by tolerized spleen cells into the naive cell culture system. Because the doses and experimental procedures used were comparable to those commonly used for suppressor cell generation and assay, it is suggested that antigen-specific suppressor cells may produce their effects through passive transfer of antigen and/or tolerogen.",
author = "Dintzis, {R. Z.} and Middleton, {M. H.} and Dintzis, {Howard M}",
year = "1988",
language = "English (US)",
volume = "28",
pages = "747--757",
journal = "Scandinavian Journal of Immunology",
issn = "0300-9475",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Tolerogen-mediated suppression of the immune response. Suppressor cells as passive transfer agents

AU - Dintzis, R. Z.

AU - Middleton, M. H.

AU - Dintzis, Howard M

PY - 1988

Y1 - 1988

N2 - The anti-dinitro-phenyl (Dnp) IgM antibody response in mice was inhibited by administration of either a non-immunogenic form of Dnp-polyacrylamide (Dnp-Pa) or an excess amount of an immunogenic form of Dnp-Pa. Spleen cells, alive or heat-killed, from mice tolerized in vivo by either method, inhibited the anti-Dnp response of naive spleen cells co-cultured in vitro with antigen. Conversely, donor cells tolerized in vivo by a high dose of immunogenic Dnp-Pa, when titrated into a naive cell culture which contained no antigen, produced a stimulatory dose-response curve. Both the dose-dependent inhibition and stimulation correlated strongly with the amount of 125I-labelled Dnp-Pa carried over by tolerized spleen cells into the naive cell culture system. Because the doses and experimental procedures used were comparable to those commonly used for suppressor cell generation and assay, it is suggested that antigen-specific suppressor cells may produce their effects through passive transfer of antigen and/or tolerogen.

AB - The anti-dinitro-phenyl (Dnp) IgM antibody response in mice was inhibited by administration of either a non-immunogenic form of Dnp-polyacrylamide (Dnp-Pa) or an excess amount of an immunogenic form of Dnp-Pa. Spleen cells, alive or heat-killed, from mice tolerized in vivo by either method, inhibited the anti-Dnp response of naive spleen cells co-cultured in vitro with antigen. Conversely, donor cells tolerized in vivo by a high dose of immunogenic Dnp-Pa, when titrated into a naive cell culture which contained no antigen, produced a stimulatory dose-response curve. Both the dose-dependent inhibition and stimulation correlated strongly with the amount of 125I-labelled Dnp-Pa carried over by tolerized spleen cells into the naive cell culture system. Because the doses and experimental procedures used were comparable to those commonly used for suppressor cell generation and assay, it is suggested that antigen-specific suppressor cells may produce their effects through passive transfer of antigen and/or tolerogen.

UR - http://www.scopus.com/inward/record.url?scp=0024217350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024217350&partnerID=8YFLogxK

M3 - Article

C2 - 2976522

AN - SCOPUS:0024217350

VL - 28

SP - 747

EP - 757

JO - Scandinavian Journal of Immunology

JF - Scandinavian Journal of Immunology

SN - 0300-9475

IS - 6

ER -