Tn7 transposition: two transposition pathways directed by five Tn7-encoded genes.

C. S. Waddell, N. L. Craig

Research output: Contribution to journalArticlepeer-review

Abstract

The bacterial transposon Tn7 is capable of high-frequency transposition to a specific site in the Escherichia coli chromosome, attTn7, and of low-frequency transposition to sites other than attTn7. Using an in vitro insertional mutagenesis procedure, we have identified and characterized five tns (Tn seven) genes that are essential for Tn7 transposition. Three of these genes, tnsA, tnsB, and tnsC, are required, but are not sufficient, for all Tn7 transposition events. In addition, tnsD is specifically required for transposition to attTn7, whereas tnsE is specifically required for transposition to other sites. Thus, Tn7 is an elaborate transposon that encodes two distinct but overlapping transposition pathways.

Original languageEnglish (US)
Pages (from-to)137-149
Number of pages13
JournalGenes & development
Volume2
Issue number2
DOIs
StatePublished - Feb 1988
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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