@article{d20437e6e48c424eb6c2b72cfa7f0eb9,
title = "Tn7 transposition: Target DNA recognition is mediated by multiple Tn7-encoded proteins in a purified in vitro system",
abstract = "We have reconstituted the transposition of the bacterial transposon TO into its specific insertion site attTn7 with four purified Tn7-encoded proteins, TnsA+TnsB+ TnsC+TnsD, and ATP. TnsA+TnsB+TnsC form a {"}core{"} recombination machine that recognizes the transposon ends and executes DNA breakage and joining; TnsD specifically recognizes attTn7. TnsA+TnsB+TnsC are specifically targeted to attTn7 through the TnsD-dependent interaction of TnsC, a nonspecific DNA-binding protein, with attTn7. Recombination appears to be activated by the assembly of a nucleoprotein complex containing the DNA substrates and Tns proteins. We suggest that TnsC plays a central role in communication between the transposon and the target DNA, particularly in directing insertion away from DNAs already containing a copy of Tn7.",
author = "Bainton, {Roland J.} and Kubo, {Kenneth M.} and Feng, {Jian nong} and Craig, {Nancy L.}",
note = "Funding Information: The first two authors contributed equally to this work. We are pleased to thank Margie Lopata for her excellent technical assistance. We thank Bruce Alberts. Sandy Johnson, Rick Myers, and members of the laboratory, in particular Anne Stellwagen, for advice and comments on the manuscript. R. J. 8. was supported by funds from the Medical ScientistTraining Program, MerckSharpand DoehmeResearch Laboratories, the University of California at San Francisco (UCSF) Department of Biochemistry Tompkins Memorial Fund, and the UCSF Program in Biological Sciences. K. M. K. was supported by funds from the UCSF Genetics Training Grant. The work was supported by a grant from the National Institute of Allergy and Infectious Diseases to N. L. C. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",
year = "1993",
month = mar,
day = "26",
doi = "10.1016/0092-8674(93)90581-A",
language = "English (US)",
volume = "72",
pages = "931--943",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "6",
}