Tn7 transposition: Recognition of the attTn7 target sequence

C. S. Waddell, N. L. Craig

Research output: Contribution to journalArticle

Abstract

The bacterial transposon Tn7 encodes two distinct but overlapping transposition pathways. tnsABC + tnsD promote transposition to a specific site, attTn7, while tnsABC + tnsE promote transposition to many other sites unrelated to attTn7. We have identified a tnsD-dependent DNA binding activity that specifically recognizes attTn7. We have localized the recognition sequences for this activity to a 28-base-pair region and have shown that this same region can provide specific properties of an attTn7 target in vivo. Interestingly, these sequences are positioned more than 25 base pairs from the specific point of Tn7 insertion.

Original languageEnglish (US)
Pages (from-to)3958-3962
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number11
DOIs
StatePublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

  • General

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