TMPRSS2-ERG gene fusion status in minute (minimal) prostatic adenocarcinoma

Roula Albadine, Mathieu Latour, Antoun Toubaji, Michael Haffner, William B Isaacs, Elizabeth A Platz, Alan Keith Meeker, Angelo Michael Demarzo, Jonathan Ira Epstein, George J. Netto

Research output: Contribution to journalArticle

Abstract

Minute prostatic adenocarcinomas are considered to be of insufficient virulence. Given recent suggestions of TMPRSS2-ERG gene fusion association with aggressive prostatic adenocarcinoma, we evaluated the incidence of TMPRSS2-ERG fusion in minute prostatic adenocarcinomas. A total of 45 consecutive prostatectomies with minute adenocarcinoma were used for tissue microarray construction. A total of 63 consecutive non-minimal, Gleason Score 6 tumors, from a separate PSA Era prostatectomy tissue microarray, were used for comparison. FISH was carried out using ERG break-apart probes. Tumors were assessed for fusion by deletion (Edel) or split (Esplit), duplicated fusions and low-level copy number gain in normal ERG gene locus. Minute adenocarcinomas: Fusion was evaluable in 32/45 tumors (71%). Fifteen out of 32 (47%) tumors were positive for fusion. Six (19%) were of the Edel class and 7 (22%) were classified as combined EdelEsplit. Non-minute adenocarcinomas (pT2): Fusion was identified in 20/30 tumors (67%). Four (13%) were of Edel class and 5 (17%) were combined EdelEsplit. Duplicated fusions were encountered in 5 (16%) tumors. Non-minute adenocarcinomas (pT3): Fusion was identified in 19/33 (58%). Fusion was due to a deletion in 6 (18%) tumors. Seven tumors (21%) were classified as combined EdelEsplit. One tumor showed Esplit alone. Duplicated fusions were encountered in 3 (9%) cases. The incidence of duplicated fusions was higher in non-minute adenocarcinomas (13 vs 0%; P0.03). A trend for higher incidence of low-level copy number gain in normal ERG gene locus without fusion was noted in non-minute adenocarcinomas (10 vs 0%; P0.07). We found a TMPRSS2-ERG fusion rate of 47% in minute adenocarcinomas. The latter is not significantly different from that of grade matched non-minute adenocarcinomas. The incidence of duplicated fusion was higher in non-minute adenocarcinomas. Our finding of comparable rate of TMPRSS2-ERG fusion in minute adenocarcinomas may argue against its value as a marker of aggressive prostate carcinoma phenotype.

Original languageEnglish (US)
Pages (from-to)1415-1422
Number of pages8
JournalModern Pathology
Volume22
Issue number11
DOIs
StatePublished - Nov 2009

Fingerprint

Gene Fusion
Adenocarcinoma
Neoplasms
Incidence
Prostatectomy
Neoplasm Grading
Genes
Virulence
Prostate

Keywords

  • Minute prostatic adenocarcinoma
  • TMPRSS2-ERG fusion

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

TMPRSS2-ERG gene fusion status in minute (minimal) prostatic adenocarcinoma. / Albadine, Roula; Latour, Mathieu; Toubaji, Antoun; Haffner, Michael; Isaacs, William B; Platz, Elizabeth A; Meeker, Alan Keith; Demarzo, Angelo Michael; Epstein, Jonathan Ira; Netto, George J.

In: Modern Pathology, Vol. 22, No. 11, 11.2009, p. 1415-1422.

Research output: Contribution to journalArticle

Albadine, Roula ; Latour, Mathieu ; Toubaji, Antoun ; Haffner, Michael ; Isaacs, William B ; Platz, Elizabeth A ; Meeker, Alan Keith ; Demarzo, Angelo Michael ; Epstein, Jonathan Ira ; Netto, George J. / TMPRSS2-ERG gene fusion status in minute (minimal) prostatic adenocarcinoma. In: Modern Pathology. 2009 ; Vol. 22, No. 11. pp. 1415-1422.
@article{b025e663ea9b4751a282ae97a88acd8c,
title = "TMPRSS2-ERG gene fusion status in minute (minimal) prostatic adenocarcinoma",
abstract = "Minute prostatic adenocarcinomas are considered to be of insufficient virulence. Given recent suggestions of TMPRSS2-ERG gene fusion association with aggressive prostatic adenocarcinoma, we evaluated the incidence of TMPRSS2-ERG fusion in minute prostatic adenocarcinomas. A total of 45 consecutive prostatectomies with minute adenocarcinoma were used for tissue microarray construction. A total of 63 consecutive non-minimal, Gleason Score 6 tumors, from a separate PSA Era prostatectomy tissue microarray, were used for comparison. FISH was carried out using ERG break-apart probes. Tumors were assessed for fusion by deletion (Edel) or split (Esplit), duplicated fusions and low-level copy number gain in normal ERG gene locus. Minute adenocarcinomas: Fusion was evaluable in 32/45 tumors (71{\%}). Fifteen out of 32 (47{\%}) tumors were positive for fusion. Six (19{\%}) were of the Edel class and 7 (22{\%}) were classified as combined EdelEsplit. Non-minute adenocarcinomas (pT2): Fusion was identified in 20/30 tumors (67{\%}). Four (13{\%}) were of Edel class and 5 (17{\%}) were combined EdelEsplit. Duplicated fusions were encountered in 5 (16{\%}) tumors. Non-minute adenocarcinomas (pT3): Fusion was identified in 19/33 (58{\%}). Fusion was due to a deletion in 6 (18{\%}) tumors. Seven tumors (21{\%}) were classified as combined EdelEsplit. One tumor showed Esplit alone. Duplicated fusions were encountered in 3 (9{\%}) cases. The incidence of duplicated fusions was higher in non-minute adenocarcinomas (13 vs 0{\%}; P0.03). A trend for higher incidence of low-level copy number gain in normal ERG gene locus without fusion was noted in non-minute adenocarcinomas (10 vs 0{\%}; P0.07). We found a TMPRSS2-ERG fusion rate of 47{\%} in minute adenocarcinomas. The latter is not significantly different from that of grade matched non-minute adenocarcinomas. The incidence of duplicated fusion was higher in non-minute adenocarcinomas. Our finding of comparable rate of TMPRSS2-ERG fusion in minute adenocarcinomas may argue against its value as a marker of aggressive prostate carcinoma phenotype.",
keywords = "Minute prostatic adenocarcinoma, TMPRSS2-ERG fusion",
author = "Roula Albadine and Mathieu Latour and Antoun Toubaji and Michael Haffner and Isaacs, {William B} and Platz, {Elizabeth A} and Meeker, {Alan Keith} and Demarzo, {Angelo Michael} and Epstein, {Jonathan Ira} and Netto, {George J.}",
year = "2009",
month = "11",
doi = "10.1038/modpathol.2009.121",
language = "English (US)",
volume = "22",
pages = "1415--1422",
journal = "Modern Pathology",
issn = "0893-3952",
publisher = "Nature Publishing Group",
number = "11",

}

TY - JOUR

T1 - TMPRSS2-ERG gene fusion status in minute (minimal) prostatic adenocarcinoma

AU - Albadine, Roula

AU - Latour, Mathieu

AU - Toubaji, Antoun

AU - Haffner, Michael

AU - Isaacs, William B

AU - Platz, Elizabeth A

AU - Meeker, Alan Keith

AU - Demarzo, Angelo Michael

AU - Epstein, Jonathan Ira

AU - Netto, George J.

PY - 2009/11

Y1 - 2009/11

N2 - Minute prostatic adenocarcinomas are considered to be of insufficient virulence. Given recent suggestions of TMPRSS2-ERG gene fusion association with aggressive prostatic adenocarcinoma, we evaluated the incidence of TMPRSS2-ERG fusion in minute prostatic adenocarcinomas. A total of 45 consecutive prostatectomies with minute adenocarcinoma were used for tissue microarray construction. A total of 63 consecutive non-minimal, Gleason Score 6 tumors, from a separate PSA Era prostatectomy tissue microarray, were used for comparison. FISH was carried out using ERG break-apart probes. Tumors were assessed for fusion by deletion (Edel) or split (Esplit), duplicated fusions and low-level copy number gain in normal ERG gene locus. Minute adenocarcinomas: Fusion was evaluable in 32/45 tumors (71%). Fifteen out of 32 (47%) tumors were positive for fusion. Six (19%) were of the Edel class and 7 (22%) were classified as combined EdelEsplit. Non-minute adenocarcinomas (pT2): Fusion was identified in 20/30 tumors (67%). Four (13%) were of Edel class and 5 (17%) were combined EdelEsplit. Duplicated fusions were encountered in 5 (16%) tumors. Non-minute adenocarcinomas (pT3): Fusion was identified in 19/33 (58%). Fusion was due to a deletion in 6 (18%) tumors. Seven tumors (21%) were classified as combined EdelEsplit. One tumor showed Esplit alone. Duplicated fusions were encountered in 3 (9%) cases. The incidence of duplicated fusions was higher in non-minute adenocarcinomas (13 vs 0%; P0.03). A trend for higher incidence of low-level copy number gain in normal ERG gene locus without fusion was noted in non-minute adenocarcinomas (10 vs 0%; P0.07). We found a TMPRSS2-ERG fusion rate of 47% in minute adenocarcinomas. The latter is not significantly different from that of grade matched non-minute adenocarcinomas. The incidence of duplicated fusion was higher in non-minute adenocarcinomas. Our finding of comparable rate of TMPRSS2-ERG fusion in minute adenocarcinomas may argue against its value as a marker of aggressive prostate carcinoma phenotype.

AB - Minute prostatic adenocarcinomas are considered to be of insufficient virulence. Given recent suggestions of TMPRSS2-ERG gene fusion association with aggressive prostatic adenocarcinoma, we evaluated the incidence of TMPRSS2-ERG fusion in minute prostatic adenocarcinomas. A total of 45 consecutive prostatectomies with minute adenocarcinoma were used for tissue microarray construction. A total of 63 consecutive non-minimal, Gleason Score 6 tumors, from a separate PSA Era prostatectomy tissue microarray, were used for comparison. FISH was carried out using ERG break-apart probes. Tumors were assessed for fusion by deletion (Edel) or split (Esplit), duplicated fusions and low-level copy number gain in normal ERG gene locus. Minute adenocarcinomas: Fusion was evaluable in 32/45 tumors (71%). Fifteen out of 32 (47%) tumors were positive for fusion. Six (19%) were of the Edel class and 7 (22%) were classified as combined EdelEsplit. Non-minute adenocarcinomas (pT2): Fusion was identified in 20/30 tumors (67%). Four (13%) were of Edel class and 5 (17%) were combined EdelEsplit. Duplicated fusions were encountered in 5 (16%) tumors. Non-minute adenocarcinomas (pT3): Fusion was identified in 19/33 (58%). Fusion was due to a deletion in 6 (18%) tumors. Seven tumors (21%) were classified as combined EdelEsplit. One tumor showed Esplit alone. Duplicated fusions were encountered in 3 (9%) cases. The incidence of duplicated fusions was higher in non-minute adenocarcinomas (13 vs 0%; P0.03). A trend for higher incidence of low-level copy number gain in normal ERG gene locus without fusion was noted in non-minute adenocarcinomas (10 vs 0%; P0.07). We found a TMPRSS2-ERG fusion rate of 47% in minute adenocarcinomas. The latter is not significantly different from that of grade matched non-minute adenocarcinomas. The incidence of duplicated fusion was higher in non-minute adenocarcinomas. Our finding of comparable rate of TMPRSS2-ERG fusion in minute adenocarcinomas may argue against its value as a marker of aggressive prostate carcinoma phenotype.

KW - Minute prostatic adenocarcinoma

KW - TMPRSS2-ERG fusion

UR - http://www.scopus.com/inward/record.url?scp=70350686099&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70350686099&partnerID=8YFLogxK

U2 - 10.1038/modpathol.2009.121

DO - 10.1038/modpathol.2009.121

M3 - Article

VL - 22

SP - 1415

EP - 1422

JO - Modern Pathology

JF - Modern Pathology

SN - 0893-3952

IS - 11

ER -