TLR9 is critical for glioma stem cell maintenance and targeting

Andreas Herrmann, Gregory Cherryholmes, Anne Schroeder, Jillian Phallen, Darya Alizadeh, Hong Xin, Tianyi Wang, Heehyoung Lee, Christoph Lahtz, Piotr Swiderski, Brian Armstrong, Claudia Kowolik, Gary L. Gallia, Michael Lim, Christine Brown, Behnam Badie, Stephen Forman, Marcin Kortylewski, Richard Jove, Hua Yu

Research output: Contribution to journalArticlepeer-review

Abstract

Understanding supports for cancer stem-like cells in malignant glioma may suggest therapeutic strategies for their elimination. Here, we show that the Toll-like receptor TLR9 is elevated in glioma stem-like cells (GSC) in which it contributes to glioma growth. TLR9 overexpression is regulated by STAT3, which is required for GSC maintenance. Stimulation of TLR9 with a CpG ligand (CpG ODN) promoted GSC growth, whereas silencing TLR9 expression abrogated GSC development. CpG-ODN treatment induced Frizzled4-dependent activation of JAK2, thereby activating STAT3. Targeted delivery of siRNA into GSC was achieved via TLR9 using CpG-siRNA conjugates. Through local or systemic treatment, administration of CpG-Stat3 siRNA to silence STAT3 in vivo reduced GSC along with glioma growth. Our findings identify TLR9 as a functional marker for GSC and a target for the delivery of efficacious therapeutics for glioma treatment.

Original languageEnglish (US)
Pages (from-to)5218-5228
Number of pages11
JournalCancer Research
Volume74
Issue number18
DOIs
StatePublished - Jul 21 2014

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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