TLR9- and FcεRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression

John Thomas Schroeder, Anja P. Bieneman, Huiqing Xiao, Kristin L. Chichester, Kavitha Vasagar, Sarbjit S Saini, Mark Chang Hwa Liu

Research output: Contribution to journalArticle

Abstract

Plasmacytoid dendritic cells (pDC) express not only TLR9 molecules through which ligation with CpG DNA favors Th1 responses but also possess IgE receptors (FcεRI) implicated in allergen presentation and induction of Th2 responses. This dichotomy prompted an investigation to determine whether TLR9- and IgE receptor-mediated responses oppose one another in pDC by affecting receptor expression and associated functional responses. Results showed that IgE cross-linking reduced TLR9 in pDC and inhibited the capacity of these cells to secrete IFN-α when stimulated with the CpG oligodeoxynucleotide (ODN)-2216. In contrast, an ∼15-fold reduction in FcεRIα mRNA and a loss in surface protein were seen in pDC first exposed to TLR9 ligation with ODN-2216. Results indicated that type I IFNs partly mediated this effect, as rIFN-α also caused a significant ∼4-fold reduction in FcεRIα mRNA. Finally, this reduction in FcεRIα mediated by ODN-2216 correlated with a selective suppression of allergen-induced CD4 + T cell proliferation, but not of responses resulting from tetanos toxoid. Overall, these results imply mechanisms by which specific innate and IgE-dependent immune responses counterregulate one another at the dendritic cell level and may have significant impact on whether an ensuing response is either of Th1 or Th2 in nature.

Original languageEnglish (US)
Pages (from-to)5724-5731
Number of pages8
JournalJournal of Immunology
Volume175
Issue number9
StatePublished - Nov 1 2005

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Dendritic Cells
Oligodeoxyribonucleotides
IgE Receptors
Allergens
Immunoglobulin E
Ligation
Toll-Like Receptor 9
Toxoids
Messenger RNA
Membrane Proteins
Cell Proliferation
T-Lymphocytes
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

TLR9- and FcεRI-mediated responses oppose one another in plasmacytoid dendritic cells by down-regulating receptor expression. / Schroeder, John Thomas; Bieneman, Anja P.; Xiao, Huiqing; Chichester, Kristin L.; Vasagar, Kavitha; Saini, Sarbjit S; Liu, Mark Chang Hwa.

In: Journal of Immunology, Vol. 175, No. 9, 01.11.2005, p. 5724-5731.

Research output: Contribution to journalArticle

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AU - Chichester, Kristin L.

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AU - Saini, Sarbjit S

AU - Liu, Mark Chang Hwa

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