TLR2-mediated activation of neutrophils in response to German cockroach frass

Kristen Page, Kristin M. Lierl, Valerie S. Hughes, Ping Zhou, John R. Ledford, Marsha Wills-Karp

Research output: Contribution to journalArticle

Abstract

It is becoming increasingly clear that innate immune mediators play a role in regulating adaptive immune responses in asthma pathogenesis. Cockroach exposure is a major risk factor for the development of asthma. In this study we asked whether German cockroach (GC) feces (frass) could initiate an innate immune response. Naive BALB/c mice were challenged with a single intratracheal inhalation of GC frass. Proinflammatory cytokines were significantly increased in the bronchoalveolar lavage fluid at 3 h and were maintained at higher than baseline levels for at least 24 h. Neutrophil migration into the airways was evident as early as 3 h but was maximal between 6 and 24 h postinhalation. The early increase in cytokine expression was independent of TLR2 or TLR4. Newly infiltrated airway neutrophils were responsible for maintaining high levels of cytokines in the airways. Using neutrophils as an early marker of the innate immune response, we show that show that neutrophils isolated from the airways following GC frass inhalation express TLR2 and release cytokines. GC frass directly affected neutrophil cytokine production via TLR2, but not TLR4, as evidenced by the use of TLR-neutralizing Abs and neutrophils from TLR-deficient mice. Activation of cytokine expression occurred via GC frass-induced NF-κB translocation and DNA binding. These data show that GC frass contains a TLR2 agonist and, to our knowledge, this is the first report of an allergen directly activating cells of the innate immune system via TLR2 and suggests an important link between innate and adaptive immunity.

Original languageEnglish (US)
Pages (from-to)6317-6324
Number of pages8
JournalJournal of Immunology
Volume180
Issue number9
StatePublished - May 1 2008
Externally publishedYes

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Blattellidae
Neutrophil Activation
Neutrophils
Cytokines
Innate Immunity
Adaptive Immunity
Inhalation
Asthma
Cockroaches
Bronchoalveolar Lavage Fluid
Feces
Allergens
Immune System
DNA

ASJC Scopus subject areas

  • Immunology

Cite this

Page, K., Lierl, K. M., Hughes, V. S., Zhou, P., Ledford, J. R., & Wills-Karp, M. (2008). TLR2-mediated activation of neutrophils in response to German cockroach frass. Journal of Immunology, 180(9), 6317-6324.

TLR2-mediated activation of neutrophils in response to German cockroach frass. / Page, Kristen; Lierl, Kristin M.; Hughes, Valerie S.; Zhou, Ping; Ledford, John R.; Wills-Karp, Marsha.

In: Journal of Immunology, Vol. 180, No. 9, 01.05.2008, p. 6317-6324.

Research output: Contribution to journalArticle

Page, K, Lierl, KM, Hughes, VS, Zhou, P, Ledford, JR & Wills-Karp, M 2008, 'TLR2-mediated activation of neutrophils in response to German cockroach frass', Journal of Immunology, vol. 180, no. 9, pp. 6317-6324.
Page K, Lierl KM, Hughes VS, Zhou P, Ledford JR, Wills-Karp M. TLR2-mediated activation of neutrophils in response to German cockroach frass. Journal of Immunology. 2008 May 1;180(9):6317-6324.
Page, Kristen ; Lierl, Kristin M. ; Hughes, Valerie S. ; Zhou, Ping ; Ledford, John R. ; Wills-Karp, Marsha. / TLR2-mediated activation of neutrophils in response to German cockroach frass. In: Journal of Immunology. 2008 ; Vol. 180, No. 9. pp. 6317-6324.
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