Tissue-specific regulation of avian vitamin D-dependent calcium-binding protein 28-kDa mRNA by 1,25-dihydroxyvitamin D3

Thomas Clemens, S. A. McGlade, K. P. Garrett, N. Horiuchi, G. N. Hendy

Research output: Contribution to journalArticle

Abstract

We have studied the regulation, by 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3), of vitamin D-dependent calcium-binding protein (28-kDa CaBP) mRNA in chick tissues in vivo. Northern analysis of poly(A)+ RNA was carried out using, as hybridization probes, synthetic oligonucleotides complementary to chick 28-kDa CaBP mRNA. In vitamin D-deficient chicks, 28-kDa CaBP mRNA was virtually undetectable in intestine, was clearly detectable in kidney, and present at the highest levels in cerebellum. After a single intravenous dose of 500 ng of 1,25-(OH)2D3, intestinal 28-kDa CaBP mRNA levels were increased 50-fold, kidney levels were increased 4-fold, and cerebellum levels were unchanged. Increased levels of 28-kDa CaBP mRNA were appreciated 2 h after induction and were maximal at 12 h. Pretreatment of vitamin D-deficient chicks with actinomycin D had little effect on the acute phase of the 1,25-(OH)2D3 induction of 28-kDa CaBP mRNA in intestine but blunted the induction in kidney. Pretreatment with cycloheximide caused a delayed response to 1,25-(OH)2D3 in the intestine, although control (noninhibition) levels of 28-kDa CaBP mRNA were present 12 h after hormone administration. By contrast, in the kidney, cycloheximide pretreatment resulted in an increased steady-state (vitamin D-deficient) level of 28-kDa CaBP mRNA, but completely abolished the induction of 1,25-(OH)2D3. Our studies indicate that, whereas 1,25-(OH)2D3 does not regulate 28-kDa CaBP mRNA levels in the brain, the hormone modulates 28-kDa CaBP gene expression in intestine and kidney in a tissue-specific manner, by acting through both transcriptional and post-transcriptional mechanisms.

Original languageEnglish (US)
Pages (from-to)13112-13116
Number of pages5
JournalJournal of Biological Chemistry
Volume263
Issue number26
StatePublished - 1988
Externally publishedYes

Fingerprint

S100 Calcium Binding Protein G
Calcitriol
Tissue
Messenger RNA
Intestines
Kidney
Vitamin D
Cycloheximide
Cerebellum
Hormones
Oligonucleotide Probes
Level control
Dactinomycin
Gene expression
Oligonucleotides
Brain

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tissue-specific regulation of avian vitamin D-dependent calcium-binding protein 28-kDa mRNA by 1,25-dihydroxyvitamin D3. / Clemens, Thomas; McGlade, S. A.; Garrett, K. P.; Horiuchi, N.; Hendy, G. N.

In: Journal of Biological Chemistry, Vol. 263, No. 26, 1988, p. 13112-13116.

Research output: Contribution to journalArticle

Clemens, Thomas ; McGlade, S. A. ; Garrett, K. P. ; Horiuchi, N. ; Hendy, G. N. / Tissue-specific regulation of avian vitamin D-dependent calcium-binding protein 28-kDa mRNA by 1,25-dihydroxyvitamin D3. In: Journal of Biological Chemistry. 1988 ; Vol. 263, No. 26. pp. 13112-13116.
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