Tissue specific expression of p422 protein, a putative lipid carrier, in mouse adipocytes

David A. Bernlohr; Tamara L. Doering; Thomas Kelly; M. Daniel Lane

doi:10.1016/0006-291X(85)91209-4

Tissue specific expression of p422 protein, a putative lipid carrier, in mouse adipocytes

David A. Bernlohr, Tamara L. Doering, Thomas Kelly, M. Daniel Lane

School of Medicine

Research output: Contribution to journal › Article

Abstract

The differentiation of 3T3-L1 preadipocytes leads to the expression of a new protein, p422, and its mRNA. This protein has 70% and 20-30% amino acid sequence homology to myelin P2 and the fatty acid binding proteins of liver and intestine, respectively. Investigation of the distribution in mouse tissues of p422 protein by immunoblotting and of p422 mRNA by cDNA hybridization indicates that they are expressed only in adipose tissue. Liver and intestinal fatty acid binding protein mRNA's were not detectable in mouse adipose tissue or in 3T3-L1 adipocytes. It is suggested that p422 functions as an adipocyte fatty acid binding protein.

Original language	English (US)
Pages (from-to)	850-855
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	132
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(85)91209-4
Publication status	Published - Oct 30 1985

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ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Bernlohr, D. A., Doering, T. L., Kelly, T., & Lane, M. D. (1985). Tissue specific expression of p422 protein, a putative lipid carrier, in mouse adipocytes. Biochemical and Biophysical Research Communications, 132(2), 850-855. https://doi.org/10.1016/0006-291X(85)91209-4

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AB - The differentiation of 3T3-L1 preadipocytes leads to the expression of a new protein, p422, and its mRNA. This protein has 70% and 20-30% amino acid sequence homology to myelin P2 and the fatty acid binding proteins of liver and intestine, respectively. Investigation of the distribution in mouse tissues of p422 protein by immunoblotting and of p422 mRNA by cDNA hybridization indicates that they are expressed only in adipose tissue. Liver and intestinal fatty acid binding protein mRNA's were not detectable in mouse adipose tissue or in 3T3-L1 adipocytes. It is suggested that p422 functions as an adipocyte fatty acid binding protein.

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10.1016/0006-291X(85)91209-4