Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma

Jong Lyel Roh; William H. Westra; Joseph A. Califano; David Sidransky; Wayne M. Koch

doi:10.1002/hed.21982

Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma

Jong Lyel Roh, William H. Westra, Joseph A. Califano, David Sidransky, Wayne M. Koch

School of Medicine

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Background. Tissue imprinting can generate molecular marker maps of tumor cells at deep surgical margins. The purpose of this study was to evaluate the feasibility of this method for detection of residual head and neck squamous cell carcinoma (HNSCC). Methods. Paired fresh tissue and nitrocellulose membrane imprints of tumor and deep margins were collected from 17 HNSCC resections. DNA was amplified using quantitative methylation-specific polymerase chain reaction (qMSP) for p16, DCC, KIF1A, and EDNRB. Levels of methylation in tumors and deep margins were compared. Results. DNA from imprints was adequate for qMSP. Hypermethylation of target genes was present in 12 of 17 tumors and in 8 deep margins. Methylation level was better from margin imprints than tissue. During follow-up (median, 13 months), local or regional recurrences occurred in 6 cases of which 5 had molecularly positive margins. Conclusion. Tissue imprinting is feasible for molecular detection of residual tumor at deep surgical margins and may correlate with locoregional recurrence.

Original language	English (US)
Pages (from-to)	1529-1536
Number of pages	8
Journal	Head and Neck
Volume	34
Issue number	11
DOIs	https://doi.org/10.1002/hed.21982
State	Published - Nov 2012

Keywords

deep surgical margin
head and neck squamous cell carcinoma
molecular diagnosis
quantitative methylation-specific PCR
tissue imprint

ASJC Scopus subject areas

Otorhinolaryngology

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10.1002/hed.21982

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@article{ef8961f5115e44a4843faeaf1982930d,

title = "Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma",

abstract = "Background. Tissue imprinting can generate molecular marker maps of tumor cells at deep surgical margins. The purpose of this study was to evaluate the feasibility of this method for detection of residual head and neck squamous cell carcinoma (HNSCC). Methods. Paired fresh tissue and nitrocellulose membrane imprints of tumor and deep margins were collected from 17 HNSCC resections. DNA was amplified using quantitative methylation-specific polymerase chain reaction (qMSP) for p16, DCC, KIF1A, and EDNRB. Levels of methylation in tumors and deep margins were compared. Results. DNA from imprints was adequate for qMSP. Hypermethylation of target genes was present in 12 of 17 tumors and in 8 deep margins. Methylation level was better from margin imprints than tissue. During follow-up (median, 13 months), local or regional recurrences occurred in 6 cases of which 5 had molecularly positive margins. Conclusion. Tissue imprinting is feasible for molecular detection of residual tumor at deep surgical margins and may correlate with locoregional recurrence.",

keywords = "deep surgical margin, head and neck squamous cell carcinoma, molecular diagnosis, quantitative methylation-specific PCR, tissue imprint",

author = "Roh, {Jong Lyel} and Westra, {William H.} and Califano, {Joseph A.} and David Sidransky and Koch, {Wayne M.}",

year = "2012",

month = nov,

doi = "10.1002/hed.21982",

language = "English (US)",

volume = "34",

pages = "1529--1536",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "Wiley-Liss Inc.",

number = "11",

}

TY - JOUR

T1 - Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma

AU - Roh, Jong Lyel

AU - Westra, William H.

AU - Califano, Joseph A.

AU - Sidransky, David

AU - Koch, Wayne M.

PY - 2012/11

Y1 - 2012/11

N2 - Background. Tissue imprinting can generate molecular marker maps of tumor cells at deep surgical margins. The purpose of this study was to evaluate the feasibility of this method for detection of residual head and neck squamous cell carcinoma (HNSCC). Methods. Paired fresh tissue and nitrocellulose membrane imprints of tumor and deep margins were collected from 17 HNSCC resections. DNA was amplified using quantitative methylation-specific polymerase chain reaction (qMSP) for p16, DCC, KIF1A, and EDNRB. Levels of methylation in tumors and deep margins were compared. Results. DNA from imprints was adequate for qMSP. Hypermethylation of target genes was present in 12 of 17 tumors and in 8 deep margins. Methylation level was better from margin imprints than tissue. During follow-up (median, 13 months), local or regional recurrences occurred in 6 cases of which 5 had molecularly positive margins. Conclusion. Tissue imprinting is feasible for molecular detection of residual tumor at deep surgical margins and may correlate with locoregional recurrence.

AB - Background. Tissue imprinting can generate molecular marker maps of tumor cells at deep surgical margins. The purpose of this study was to evaluate the feasibility of this method for detection of residual head and neck squamous cell carcinoma (HNSCC). Methods. Paired fresh tissue and nitrocellulose membrane imprints of tumor and deep margins were collected from 17 HNSCC resections. DNA was amplified using quantitative methylation-specific polymerase chain reaction (qMSP) for p16, DCC, KIF1A, and EDNRB. Levels of methylation in tumors and deep margins were compared. Results. DNA from imprints was adequate for qMSP. Hypermethylation of target genes was present in 12 of 17 tumors and in 8 deep margins. Methylation level was better from margin imprints than tissue. During follow-up (median, 13 months), local or regional recurrences occurred in 6 cases of which 5 had molecularly positive margins. Conclusion. Tissue imprinting is feasible for molecular detection of residual tumor at deep surgical margins and may correlate with locoregional recurrence.

KW - deep surgical margin

KW - head and neck squamous cell carcinoma

KW - molecular diagnosis

KW - quantitative methylation-specific PCR

KW - tissue imprint

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Tissue imprint for molecular mapping of deep surgical margins in patients with head and neck squamous cell carcinoma

Abstract

Keywords

ASJC Scopus subject areas

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