Tissue-engineered vascular grafts: does cell seeding matter?

Tamar L. Mirensky, Narutoshi Hibino, Rajendra F. Sawh-Martinez, Tai Yi, Gustavo Villalona, Toshiharu Shinoka, Christopher K. Breuer

Research output: Contribution to journalArticle

Abstract

Purpose: Use of tissue-engineered vascular grafts (TEVGs) in the repair of congenital heart defects provides growth and remodeling potential. Little is known about the mechanisms involved in neovessel formation. We sought to define the role of seeded monocytes derived from bone marrow mononuclear cells (BM-MNCs) on neovessel formation. Methods: Small diameter biodegradable tubular scaffolds were constructed. Scaffolds were seeded with the entire population of BM-MNC (n = 15), BM-MNC excluding monocytes (n = 15), or only monocytes (n = 15) and implanted as infrarenal inferior vena cava (IVC) interposition grafts into severe combined immunodeficiency/bg mice. Grafts were evaluated at 1 week, 10 weeks, or 6 months via ultrasonography and microcomputed tomography, as well as by histologic and immunohistochemical techniques. Results: All grafts remained patent without stenosis or aneurysm formation. Neovessels contained a luminal endothelial lining surrounded by concentric smooth muscle cell layer and collagen similar to that seen in the native mouse IVC. Graft diameters differed significantly between those scaffolds seeded with only monocytes (1.022 ± 0.155 mm) and those seeded without monocytes (0.771 ± 0.121 mm; P = .021) at 6 months. Conclusions: Monocytes may play a role in maintaining graft patency. Incorporation of such findings into the development of second-generation TEVGs will promote graft patency and success.

Original languageEnglish (US)
Pages (from-to)1299-1305
Number of pages7
JournalJournal of Pediatric Surgery
Volume45
Issue number6
DOIs
StatePublished - 2010
Externally publishedYes

Keywords

  • Cell seeding
  • Mononuclear cells
  • Tissue-engineering
  • Vascular conduit
  • Vascular graft

ASJC Scopus subject areas

  • Surgery
  • Pediatrics, Perinatology, and Child Health
  • Medicine(all)

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