Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea

Anirudha Singh, David Lee, Harrison Jeong, Christine Yu, Jiuru Li, Chen Hao Fang, Praveena Sabnekar, Xiaopu Liu, Takahiro Yoshida, Nikolai Sopko, Trinity Bivalacqua

Research output: Contribution to journalArticle

Abstract

Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea, as well as other tubular organs. A Neo-Urinary Conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed. In this study, we report our findings on the feasibility of using a decellularized trachea for NUC applications. As a NUC scaffold, decellularized trachea provides benefits of having not only naturally occurring biological components but also having sufficient mechanical properties and structural integrity. We, therefore, decellularized rabbit trachea, evaluated its mechanical performance, and investigated its ability to support in vitro growth of human smooth muscle cells (hSMCs) and human urothelial cells (hUCs). The decellularized trachea had appropriate biomechanical properties with ultimate tensile strength of ∼0.34 MPa in longitudinal direction and ∼1.0 MPa in circumferential direction and resisted a radial burst pressure of >155 mm Hg. Cell morphology study by scanning electron microscopy further showed that hUCs grown on decellularized trachea adopted a typical flatten and interconnected network structure in the lumen of the scaffold, while they formed a round spherical shape and did not spread on the outer surfaces. SMCs, on the other hand, spread well throughout the scaffold. The gene expression analysis by real time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence studies further confirmed scaffold's ability to support long-term growth of hSMCs. Since uroepithelium has been shown to regenerate itself over time in vivo, these findings suggest that it is possible to construct a NUC from decellularized trachea without any preseeding of UCs. In future, we plan to translate decellularized trachea in a preclinical animal model and evaluate its biological performance.

Original languageEnglish (US)
Pages (from-to)1456-1467
Number of pages12
JournalTissue Engineering - Part A
Volume24
Issue number19-20
DOIs
StatePublished - Oct 1 2018

Fingerprint

Trachea
Scaffolds
Tissue
Muscle
Cells
Mechanical properties
Polymerase chain reaction
Structural integrity
Scaffolds (biology)
Tissue engineering
Gene expression
Animals
Smooth Muscle Myocytes
Tensile strength
Urinary Bladder Diseases
Scanning electron microscopy
Urinary Diversion
Tensile Strength
Chemical analysis
Tissue Engineering

Keywords

  • bladder cancer
  • decellularized tissues
  • neo-urinary conduit
  • scaffolds
  • trachea

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomaterials
  • Biomedical Engineering

Cite this

Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea. / Singh, Anirudha; Lee, David; Jeong, Harrison; Yu, Christine; Li, Jiuru; Fang, Chen Hao; Sabnekar, Praveena; Liu, Xiaopu; Yoshida, Takahiro; Sopko, Nikolai; Bivalacqua, Trinity.

In: Tissue Engineering - Part A, Vol. 24, No. 19-20, 01.10.2018, p. 1456-1467.

Research output: Contribution to journalArticle

Singh, A, Lee, D, Jeong, H, Yu, C, Li, J, Fang, CH, Sabnekar, P, Liu, X, Yoshida, T, Sopko, N & Bivalacqua, T 2018, 'Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea', Tissue Engineering - Part A, vol. 24, no. 19-20, pp. 1456-1467. https://doi.org/10.1089/ten.tea.2017.0436
Singh, Anirudha ; Lee, David ; Jeong, Harrison ; Yu, Christine ; Li, Jiuru ; Fang, Chen Hao ; Sabnekar, Praveena ; Liu, Xiaopu ; Yoshida, Takahiro ; Sopko, Nikolai ; Bivalacqua, Trinity. / Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea. In: Tissue Engineering - Part A. 2018 ; Vol. 24, No. 19-20. pp. 1456-1467.
@article{61dc5df182064275993fed8cbc6dd48a,
title = "Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea",
abstract = "Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea, as well as other tubular organs. A Neo-Urinary Conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed. In this study, we report our findings on the feasibility of using a decellularized trachea for NUC applications. As a NUC scaffold, decellularized trachea provides benefits of having not only naturally occurring biological components but also having sufficient mechanical properties and structural integrity. We, therefore, decellularized rabbit trachea, evaluated its mechanical performance, and investigated its ability to support in vitro growth of human smooth muscle cells (hSMCs) and human urothelial cells (hUCs). The decellularized trachea had appropriate biomechanical properties with ultimate tensile strength of ∼0.34 MPa in longitudinal direction and ∼1.0 MPa in circumferential direction and resisted a radial burst pressure of >155 mm Hg. Cell morphology study by scanning electron microscopy further showed that hUCs grown on decellularized trachea adopted a typical flatten and interconnected network structure in the lumen of the scaffold, while they formed a round spherical shape and did not spread on the outer surfaces. SMCs, on the other hand, spread well throughout the scaffold. The gene expression analysis by real time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence studies further confirmed scaffold's ability to support long-term growth of hSMCs. Since uroepithelium has been shown to regenerate itself over time in vivo, these findings suggest that it is possible to construct a NUC from decellularized trachea without any preseeding of UCs. In future, we plan to translate decellularized trachea in a preclinical animal model and evaluate its biological performance.",
keywords = "bladder cancer, decellularized tissues, neo-urinary conduit, scaffolds, trachea",
author = "Anirudha Singh and David Lee and Harrison Jeong and Christine Yu and Jiuru Li and Fang, {Chen Hao} and Praveena Sabnekar and Xiaopu Liu and Takahiro Yoshida and Nikolai Sopko and Trinity Bivalacqua",
year = "2018",
month = "10",
day = "1",
doi = "10.1089/ten.tea.2017.0436",
language = "English (US)",
volume = "24",
pages = "1456--1467",
journal = "Tissue Engineering - Part A.",
issn = "1937-3341",
publisher = "Mary Ann Liebert Inc.",
number = "19-20",

}

TY - JOUR

T1 - Tissue-Engineered Neo-Urinary Conduit from Decellularized Trachea

AU - Singh, Anirudha

AU - Lee, David

AU - Jeong, Harrison

AU - Yu, Christine

AU - Li, Jiuru

AU - Fang, Chen Hao

AU - Sabnekar, Praveena

AU - Liu, Xiaopu

AU - Yoshida, Takahiro

AU - Sopko, Nikolai

AU - Bivalacqua, Trinity

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea, as well as other tubular organs. A Neo-Urinary Conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed. In this study, we report our findings on the feasibility of using a decellularized trachea for NUC applications. As a NUC scaffold, decellularized trachea provides benefits of having not only naturally occurring biological components but also having sufficient mechanical properties and structural integrity. We, therefore, decellularized rabbit trachea, evaluated its mechanical performance, and investigated its ability to support in vitro growth of human smooth muscle cells (hSMCs) and human urothelial cells (hUCs). The decellularized trachea had appropriate biomechanical properties with ultimate tensile strength of ∼0.34 MPa in longitudinal direction and ∼1.0 MPa in circumferential direction and resisted a radial burst pressure of >155 mm Hg. Cell morphology study by scanning electron microscopy further showed that hUCs grown on decellularized trachea adopted a typical flatten and interconnected network structure in the lumen of the scaffold, while they formed a round spherical shape and did not spread on the outer surfaces. SMCs, on the other hand, spread well throughout the scaffold. The gene expression analysis by real time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence studies further confirmed scaffold's ability to support long-term growth of hSMCs. Since uroepithelium has been shown to regenerate itself over time in vivo, these findings suggest that it is possible to construct a NUC from decellularized trachea without any preseeding of UCs. In future, we plan to translate decellularized trachea in a preclinical animal model and evaluate its biological performance.

AB - Decellularized tissues have been increasingly popular for constructing scaffolds for tissue engineering applications due to their beneficial biological compositions and mechanical properties. It is therefore natural to consider decellularized trachea for construction of tissue-engineered trachea, as well as other tubular organs. A Neo-Urinary Conduit (NUC) is such a tubular organ that works as a passage for urine removal in bladder cancer patients who need a urinary diversion after their diseased bladder is removed. In this study, we report our findings on the feasibility of using a decellularized trachea for NUC applications. As a NUC scaffold, decellularized trachea provides benefits of having not only naturally occurring biological components but also having sufficient mechanical properties and structural integrity. We, therefore, decellularized rabbit trachea, evaluated its mechanical performance, and investigated its ability to support in vitro growth of human smooth muscle cells (hSMCs) and human urothelial cells (hUCs). The decellularized trachea had appropriate biomechanical properties with ultimate tensile strength of ∼0.34 MPa in longitudinal direction and ∼1.0 MPa in circumferential direction and resisted a radial burst pressure of >155 mm Hg. Cell morphology study by scanning electron microscopy further showed that hUCs grown on decellularized trachea adopted a typical flatten and interconnected network structure in the lumen of the scaffold, while they formed a round spherical shape and did not spread on the outer surfaces. SMCs, on the other hand, spread well throughout the scaffold. The gene expression analysis by real time quantitative polymerase chain reaction (RT-qPCR) and immunofluorescence studies further confirmed scaffold's ability to support long-term growth of hSMCs. Since uroepithelium has been shown to regenerate itself over time in vivo, these findings suggest that it is possible to construct a NUC from decellularized trachea without any preseeding of UCs. In future, we plan to translate decellularized trachea in a preclinical animal model and evaluate its biological performance.

KW - bladder cancer

KW - decellularized tissues

KW - neo-urinary conduit

KW - scaffolds

KW - trachea

UR - http://www.scopus.com/inward/record.url?scp=85054435327&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85054435327&partnerID=8YFLogxK

U2 - 10.1089/ten.tea.2017.0436

DO - 10.1089/ten.tea.2017.0436

M3 - Article

C2 - 29649957

AN - SCOPUS:85054435327

VL - 24

SP - 1456

EP - 1467

JO - Tissue Engineering - Part A.

JF - Tissue Engineering - Part A.

SN - 1937-3341

IS - 19-20

ER -