Tissue elongation requires oscillating contractions of a basal actomyosin network

Li He, Xiaobo Wang, Ho Lam Tang, Denise J. Montell

Research output: Contribution to journalArticle

Abstract

Understanding how molecular dynamics leads to cellular behaviours that ultimately sculpt organs and tissues is a major challenge not only in basic developmental biology but also in tissue engineering and regenerative medicine. Here we use live imaging to show that the basal surfaces of Drosophila follicle cells undergo a series of directional, oscillating contractions driven by periodic myosin accumulation on a polarized actin network. Inhibition of the actomyosin contractions or their coupling to extracellular matrix (ECM) blocked elongation of the whole tissue, whereas enhancement of the contractions exaggerated it. Myosin accumulated in a periodic manner before each contraction and was regulated by the small GTPase Rho, its downstream kinase, ROCK, and cytosolic calcium. Disrupting the link between the actin cytoskeleton and the ECM decreased the amplitude and period of the contractions, whereas enhancing cell-ECM adhesion increased them. In contrast, disrupting cell-cell adhesions resulted in loss of the actin network. Our findings reveal a mechanism controlling organ shape and an experimental model for the study of the effects of oscillatory actomyosin activity within a coherent cell sheet.

Original languageEnglish (US)
Pages (from-to)1133-1142
Number of pages10
JournalNature Cell Biology
Volume12
Issue number12
DOIs
StatePublished - Dec 2010

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Actomyosin
Extracellular Matrix
Myosins
Actins
Cell-Matrix Junctions
Developmental Biology
Regenerative Medicine
Monomeric GTP-Binding Proteins
Molecular Dynamics Simulation
Tissue Engineering
Actin Cytoskeleton
Cell Adhesion
Drosophila
Theoretical Models
Phosphotransferases
Calcium

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tissue elongation requires oscillating contractions of a basal actomyosin network. / He, Li; Wang, Xiaobo; Tang, Ho Lam; Montell, Denise J.

In: Nature Cell Biology, Vol. 12, No. 12, 12.2010, p. 1133-1142.

Research output: Contribution to journalArticle

He, Li ; Wang, Xiaobo ; Tang, Ho Lam ; Montell, Denise J. / Tissue elongation requires oscillating contractions of a basal actomyosin network. In: Nature Cell Biology. 2010 ; Vol. 12, No. 12. pp. 1133-1142.
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