Tissue distribution and metabolism of the [32P]-Labeled oligodeoxynucleoside methylphosphonate-neoglycopeptide conjugate, [YEE(ah-GalNAc)3]-SMCC-AET-pUmpT(̄)7, in the mouse

Jon J. Hangeland, John E. Flesher, Scott F. Deamond, Yuan C. Lee, Paul O P Ts'o, J. James Frost

Research output: Contribution to journalArticlepeer-review

Abstract

Development of oligodeoxynucleotides (oligo-dNs) and their analogs as therapeutic agents is complicated by their low rate of transport across cellular membranes, which is required for interaction with the intracellular complementary nucleic acid sequences, and the lack of tissue-specific delivery. To overcome these obstacles, bioconjugates between cell surface receptor ligands and oligodeoxynucleoside methylphosphonates (oligo-MPs) have been constructed containing homogeneous, chemically defined covalent linkages. We have previously established that a model conjugate, [32p]-labeled [YEE(ah-GalNAc)3]-SMCC-AET-pUmpT(̄)7 (1), is delivered to Hep G2 cells in a ligand-specific manner, reaching a peak value of 26 pmol per 106 cells after 24 hours incubation at 37°C. In this work, the in vivo behavior of this conjugate is explored. Administration of this conjugate to mice via tail vein injection demonstrates rapid uptake in liver to the extent of 69.9 ± 9.9% of the injected dose after 15 minutes. Thereafter, the conjugate and its metabolites are rapidly cleared via the kidney and urine. Polyacrylamide gel electrophoresis analysis of extracts of Hep G2 cells and mouse liver reveal the conjugate 1 to be extensively metabolized. In contrast, the conjugate found in mouse urine is largely intact. These data show that this novel, biodegradable delivery vehicle represents a viable approach for the delivery of antisense oligo-MPs and other oligo-dN analogs to the liver for therapeutic and diagnostic applications.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalAntisense and Nucleic Acid Drug Development
Volume7
Issue number3
StatePublished - 1997

ASJC Scopus subject areas

  • Genetics
  • Pharmacology

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