Tissue acylation by the chlorofluorocarbon substitute 2,2-dichloro-1,1,1-trifluoroethane

James W. Harris, Lance R. Pohl, Jackie L. Martin, M. W. Anders

Research output: Contribution to journalArticlepeer-review

Abstract

Hydrochlorofluorocarbons (HCFCs) are being developed as substitutes for ozone-depleting chlorofluorocarbons (CFCs); because widespread human exposure to HCKCs may be expected, it is important to evaluate their toxicities thoroughly. Here we report studies on the bioactivation of the CFC substitute 2,2-dichloro-1,1,1-trifluoroethane (HOC-123) to an electrophilic intermediate that reacts covalently with liver proteins. HCFC-123 and its analog halothane (2-bromo-2-chloro-1,1,1-trifluoroethane) were studied in rats by 19F NMR spectroscopy, and we found that a trifluoroacetylated lysine adduct was formed with liver proteins. Also, the pattern of proteins immunoreactive with hapten-specific antitrifluoroacetylprotein antibodies was identical in livers of HCFC-123- and halothane-exposed rats. Because halothane causes an idiosyncratic, and sometimes fatal, hepatitis that is associated with an immune response against several trifluoroacetylated liver proteins, the present findings raise the possibility that humans exposed to HCFC-123 or structurally related HCFCs may be at risk of developing an immunologically mediated hepatitis.

Original languageEnglish (US)
Pages (from-to)1407-1410
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume88
Issue number4
DOIs
StatePublished - 1991

Keywords

  • F NMR
  • Hepatic metabolism
  • Hydrochlorofluorocarbons
  • Neoantigens
  • Trifluoroacetylated proteins

ASJC Scopus subject areas

  • General

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