Time-resolved fluorescent imaging in tissue

David Hattery, Victor Chernomordik, Murray Loew, Israel Gannot, Amir Gandjbakhche

Research output: Contribution to journalConference article

Abstract

Fluorescence lifetime imaging is a useful tool for quantifying site-dependent environmental conditions in tissue. Fluorophores exist with known lifetime dependencies on factors such as concentrations of O2 and other specific molecules, as well as on temperature and pH. Extracting fluorophore lifetime for deeply embedded sites in turbid media such as tissue is made difficult by the multiple scattering of photons traveling through tissue. This scattering introduces photon arrival delays that have similar characteristics to the delays resulting from the excitation and subsequent emission of photons by fluorophores. Random walk theory (RWT) provides a framework in which the two sources of diffusion-like delays can be separated so that the part due to fluorescent lifetime can be quantified. We derive a closed-form solution that predicts time-resolved photon arrivals from a deeply embedded fluorophore site. The solution requires that an average absorption coefficient be used. However, it is shown that this assumption introduces only a small error. This RWT-derived solution is also shown to be valid for a range of geometries in which the fluorophore site is embedded at least 10 mean scattering lengths and in which the fluorophore lifetime is less than 1 ns.

Original languageEnglish (US)
Pages (from-to)384-391
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume3659
Issue numberI
StatePublished - Jan 1 1999
Externally publishedYes
EventProceedings of the 1999 Medical Imaging - Physics of Medical Imaging - San Diego, CA, USA
Duration: Feb 21 1999Feb 23 1999

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Hattery, D., Chernomordik, V., Loew, M., Gannot, I., & Gandjbakhche, A. (1999). Time-resolved fluorescent imaging in tissue. Proceedings of SPIE - The International Society for Optical Engineering, 3659(I), 384-391.