Time-dependent changes in lipid metabolism in mice with methionine choline deficiency-induced fatty liver disease

Han Sol Park, Byeong Hwan Jeon, Sung Hoon Woo, Jaechan Leem, Jung Eun Jang, Min Sock Cho, In Sun Park, Ki Up Lee, Eun Hee Koh

Research output: Contribution to journalArticle

Abstract

Methionine and choline-deficient diet (MCD)-induced fatty liver is one of the best-studied animal models of fatty liver disease. The present study was performed to clarify the relative contributions of individual lipid metabolic pathways to the pathogenesis of MCD-induced fatty liver. Hepatic lipogenesis mediated by the sterol regulatory element-binding protein (SREBP-1c) was increased at 1 week, but not at 6 weeks, of MCD feeding. On the other hand, 14C-palmitate oxidation did not change at 1 week, but significantly decreased at 6 weeks. This decrease was associated with increased expression of fatty acid translocase, a key enzyme involved in fatty acid uptake. Expression of endoplasmic reticulum stress markers was increased in mice given MCD for both 1 and 6 weeks. These findings suggest the presence of time-dependent differences in lipid metabolism in MCD-induced fatty liver disease: SREBP-1c-mediated lipogenesis is important in the early stages of fatty liver disease, whereas increased fatty acid uptake and decreased fatty acid oxidation become more important in the later stages.

Original languageEnglish (US)
Pages (from-to)571-577
Number of pages7
JournalMolecules and cells
Volume32
Issue number6
DOIs
StatePublished - Dec 1 2011
Externally publishedYes

Keywords

  • Endoplasmic reticulum stress
  • Fatty acid oxidation
  • Lipogenesis
  • Non-alcholic fatty liver disease
  • Sterol regulatory element binding protein-1c

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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  • Cite this

    Park, H. S., Jeon, B. H., Woo, S. H., Leem, J., Jang, J. E., Cho, M. S., Park, I. S., Lee, K. U., & Koh, E. H. (2011). Time-dependent changes in lipid metabolism in mice with methionine choline deficiency-induced fatty liver disease. Molecules and cells, 32(6), 571-577. https://doi.org/10.1007/s10059-011-0184-6