Time course and cytokine dependence of human T-cell lymphotropic virus type 1 T-lymphocyte transformation as revealed by a microtiter infectivity assay

Deborah Persaud, J. L. Munoz, S. L. Tarsis, E. S. Parks, W. P. Parks

Research output: Contribution to journalArticle

Abstract

Human T-cell lymphotropic virus type 1 (HTLV-1) enhances the growth of T lymphocytes, allowing the generation of T-lymphocyte cell lines. This report describes a limiting-dilation assay system which uses low input numbers of HTLV-1-producing cells for generation of T-lymphocyte cultures. The HTLV-1 transformants generated with this assay system produced high levels of HTLV- 1 p24 antigen and required exogenous cytokines for maintenance. Clonal populations of CD4- or CD8-positive HTLV-1 transformants were generated with transformation efficiency rates as high as 78%. An exogenous cytokine is necessary for HTLV-1 T-lymphocyte transformation, and cytokine dependence is the most likely outcome of infection and transformation. HTLV-1 T-lymphocyte transformation can occur in the presence of cytokines other than interleukin- 2 (IL-2), such as IL-4 or IL-7. IL-4- or IL-7-dependent HTLV-1 transformants underwent T-lymphocyte mitogenesis in response to their homologous cytokines but proliferated best in the presence of IL-2. Since the receptors for IL-2, IL-4, and IL-7 share the IL-2 gamma chain, this component may be the common element in the signaling pathway for HTLV-1-associated transformation.

Original languageEnglish (US)
Pages (from-to)6297-6303
Number of pages7
JournalJournal of Virology
Volume69
Issue number10
StatePublished - 1995
Externally publishedYes

Fingerprint

Primate T-lymphotropic virus 1
Human T-lymphotropic virus 1
lymphocyte proliferation
Lymphocyte Activation
cytokines
pathogenicity
T-lymphocytes
Cytokines
T-Lymphocytes
assays
interleukin-7
interleukin-2
interleukin-4
Interleukin-7
Interleukin-4
Interleukin-2
cell lines
Interleukin-2 Receptors

ASJC Scopus subject areas

  • Immunology

Cite this

Time course and cytokine dependence of human T-cell lymphotropic virus type 1 T-lymphocyte transformation as revealed by a microtiter infectivity assay. / Persaud, Deborah; Munoz, J. L.; Tarsis, S. L.; Parks, E. S.; Parks, W. P.

In: Journal of Virology, Vol. 69, No. 10, 1995, p. 6297-6303.

Research output: Contribution to journalArticle

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