Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion

David K. Hwang, Steven M Claypool, Danielle Leuenberger, Heather L. Tienson, Carla M. Koehler

Research output: Contribution to journalArticle

Abstract

Tim54p, a component of the inner membrane TIM22 complex, does not directly mediate the import of inner membrane substrates but is required for assembly/ stability of the 300-kD TIM22 complex. In addition, Δtim54 yeast exhibit a petite-negative phenotype (also observed in yeast harboring mutations in the F1Fo ATPase, the ADP/ATP carrier, mitochondrial morphology components, or the i-AAA protease, Yme1p). Interestingly, other import mutants in our strain background are not petite-negative. We report that Tim54p is not involved in maintenance of mitochondrial DNA or mitochondrial morphology. Rather, Tim54p mediates assembly of an active Yme1p complex, after Yme1p is imported via the TIM23 pathway. Defective Yme1p assembly is likely the major contributing factor for the petite-negativity in strains lacking functional Tim54p. Thus, Tim54p has two independent functions: scaffolding/stability for the TIM22 membrane complex and assembly of Yme1p into a proteolytically active complex. As such, Tim54p links protein import, assembly, and turnover pathways in the mitochondrion.

Original languageEnglish (US)
Pages (from-to)1161-1175
Number of pages15
JournalJournal of Cell Biology
Volume178
Issue number7
DOIs
StatePublished - Sep 24 2007
Externally publishedYes

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Mitochondria
Membranes
Yeasts
ATP Translocases Mitochondrial ADP
Mitochondrial DNA
Adenosine Triphosphatases
Peptide Hydrolases
Maintenance
Phenotype
Mutation
link protein

ASJC Scopus subject areas

  • Cell Biology

Cite this

Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion. / Hwang, David K.; Claypool, Steven M; Leuenberger, Danielle; Tienson, Heather L.; Koehler, Carla M.

In: Journal of Cell Biology, Vol. 178, No. 7, 24.09.2007, p. 1161-1175.

Research output: Contribution to journalArticle

Hwang, David K. ; Claypool, Steven M ; Leuenberger, Danielle ; Tienson, Heather L. ; Koehler, Carla M. / Tim54p connects inner membrane assembly and proteolytic pathways in the mitochondrion. In: Journal of Cell Biology. 2007 ; Vol. 178, No. 7. pp. 1161-1175.
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