TY - JOUR
T1 - Tim23 - Tim50 pair coordinates functions of translocators and motor proteins in mitochondrial protein import
AU - Tamura, Yasushi
AU - Harada, Yoshihiro
AU - Shiota, Takuya
AU - Yamano, Koji
AU - Watanabe, Kazuaki
AU - Yokota, Mihoko
AU - Yamamoto, Hayashi
AU - Sesaki, Hiromi
AU - Endo, Toshiya
PY - 2009/1/12
Y1 - 2009/1/12
N2 - Mitochondrial protein traffi c requires coordinated operation of protein translocator complexes in the mitochondrial membrane. The TIM23 complex translocates and inserts proteins into the mitochondrial inner membrane. Here we analyze the intermembrane space (IMS) domains of Tim23 and Tim50, which are essential subunits of the TIM23 complex, in these functions. We fi nd that interactions of Tim23 and Tim50 in the IMS facilitate transfer of precursor proteins from the TOM40 complex, a general protein translocator in the outer membrane, to the TIM23 complex. Tim23 - Tim50 interactions also facilitate a late step of protein translocation across the inner membrane by promoting motor functions of mitochondrial Hsp70 in the matrix. Therefore, the Tim23- Tim50 pair coordinates the actions of the TOM40 and TIM23 complexes together with motor proteins for mitochondrial protein import.
AB - Mitochondrial protein traffi c requires coordinated operation of protein translocator complexes in the mitochondrial membrane. The TIM23 complex translocates and inserts proteins into the mitochondrial inner membrane. Here we analyze the intermembrane space (IMS) domains of Tim23 and Tim50, which are essential subunits of the TIM23 complex, in these functions. We fi nd that interactions of Tim23 and Tim50 in the IMS facilitate transfer of precursor proteins from the TOM40 complex, a general protein translocator in the outer membrane, to the TIM23 complex. Tim23 - Tim50 interactions also facilitate a late step of protein translocation across the inner membrane by promoting motor functions of mitochondrial Hsp70 in the matrix. Therefore, the Tim23- Tim50 pair coordinates the actions of the TOM40 and TIM23 complexes together with motor proteins for mitochondrial protein import.
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U2 - 10.1083/jcb.200808068
DO - 10.1083/jcb.200808068
M3 - Article
C2 - 19139266
AN - SCOPUS:59849090705
SN - 0021-9525
VL - 184
SP - 129
EP - 141
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 1
ER -