Tight coupling between nucleus and cell migration through the perinuclear actin cap

Dong Hwee Kim, Sangkyun Cho, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

Although eukaryotic cells are known to alternate between 'advancing' episodes of fast and persistent movement and 'hesitation' episodes of low speed and low persistence, the molecular mechanism that controls the dynamic changes in morphology, speed and persistence of eukaryotic migratory cells remains unclear. Here, we show that the movement of the interphase nucleus during random cell migration switches intermittently between two distinct modes-rotation and translocation-that follow with high fidelity the sequential rounded and elongated morphologies of the nucleus and cell body, respectively. Nuclear rotation and translocation mediate the stopand-go motion of the cell through the dynamic formation and dissolution, respectively, of the contractile perinuclear actin cap, which is dynamically coupled to the nuclear lamina and the nuclear envelope through LINC complexes. A persistent cell movement and nuclear translocation driven by the actin cap are halted following the disruption of the actin cap, which in turn allows the cell to repolarize for its next persistent move owing to nuclear rotation mediated by cytoplasmic dynein light intermediate chain 2.

Original languageEnglish (US)
Pages (from-to)2528-2541
Number of pages14
JournalJournal of Cell Science
Volume127
Issue number11
DOIs
StatePublished - 2014

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Cell Movement
Actins
Eukaryotic Cells
Cytoplasmic Dyneins
Nuclear Lamina
Dyneins
Interphase
Nuclear Envelope
Cell Body

Keywords

  • Actin cap
  • Cell migration
  • Nuclear migration

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Tight coupling between nucleus and cell migration through the perinuclear actin cap. / Kim, Dong Hwee; Cho, Sangkyun; Wirtz, Denis.

In: Journal of Cell Science, Vol. 127, No. 11, 2014, p. 2528-2541.

Research output: Contribution to journalArticle

Kim, Dong Hwee ; Cho, Sangkyun ; Wirtz, Denis. / Tight coupling between nucleus and cell migration through the perinuclear actin cap. In: Journal of Cell Science. 2014 ; Vol. 127, No. 11. pp. 2528-2541.
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