Thyroid hormone and DNA binding properties of A mutant c-erbAβ receptor associated with generalized thyroid hormone resistance

Stephen J. Usala, Fredric E. Wondisford, Tracey L. Watson, Jay B. Menke, Bruce D. Weintraub

Research output: Contribution to journalArticle

Abstract

We have previously reported a family, Kindred A, with autosomal dominant generalized thyroid hormone resistance in which affected members were found to have a mutation in the carboxy-terminal domain of the c-erbAβ thyroid hormone receptor. In the current study, the thyroid hormone and DNA-binding properties of this mutant receptor were determined using c-erbAβ protein synthesized in vitro. Both the wild-type human placental c-erbAβ and Kindred A receptors bound [125I]-triiodothyronine, although the Kindred A receptor had decreased affinity for the hormone. The affinity for triiodothyronine was 4.5 × 109 M-1 and 2.3 × 1010 M-1 for the mutant and wild-type receptors, respectively. No abnormality of DNA-binding was detected with the Kindred A receptor using a sensitive avidin-biotin DNA-binding assay with DNA fragments containing thyroid hormone response elements. The Kindred A mutant receptor which displays abnormal triiodothyronine-binding but normal DNA-binding activities in vitro acts as a dominant negative inhibitor of thyroid hormone action in man.

Original languageEnglish (US)
Pages (from-to)575-580
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume171
Issue number2
DOIs
StatePublished - Sep 14 1990

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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