Abstract
Medium conditioned by mouse thymic cells cultured for 7 days in the presence of concanavalin A (Con A) and 5% human serum enhanced erythroid colony formation by CFU-E but not BFU-E. Medium conditioned by thymic cells for less than 7 days or more than 7 days inhibited erythroid colony formation. Enhancement of erythroid colony formation occurred at both suboptimal and saturating concentrations of erythropoietin. The thymus-conditioned medium also stimulated the growth of granulocyte/macrophage colonies by itself and in the presence of a saturating concentration of WEHI-3 conditioned medium. The conditioned medium contained neither burst-promoting activity nor erythropoietin. The erythroid colony-enhancing activity in the thymus-conditioned medium was heat stable, bound to immoblized Con A, lacked protease activity and migrated with an approximate molecular weight of 68,000 on gel filtration.
Original language | English (US) |
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Pages (from-to) | 321-334 |
Number of pages | 14 |
Journal | Stem Cells |
Volume | 2 |
Issue number | 6 |
State | Published - Dec 1 1982 |
ASJC Scopus subject areas
- Molecular Medicine
- Developmental Biology
- Cell Biology