Thymocyte emigration is mediated by active movement away from stroma-derived factors

Mark C. Poznansky, Ivona T. Olszak, Richard H. Evans, Zack Z. Wang, Russell B. Foxall, Douglas P. Olson, Kathryn Weibrecht, Andrew D. Luster, David T. Scadden

Research output: Contribution to journalArticle

Abstract

T cells leave the thymus at a specific time during differentiation and do not return despite elaboration of known T cell chemoattractants by thymic stroma. We observed differentiation stage-restricted egress of thymocytes from an artificial thymus in which vascular structures or hemodynamics could not have been playing a role. Hypothesizing that active movement of cells away from a thymic product may be responsible, we demonstrated selective reduction in emigration from primary thymus by inhibitors of active movement down a concentration gradient (chemofugetaxis). Immature intrathymic precursors were insensitive to an emigration signal, whereas mature thymocytes and peripheral blood T cells were sensitive. Thymic stroma was noted to elaborate at least two proteins capable of inducing emigration, one of which was stromal cell-derived factor-1. Thymic emigration is mediated, at least in part, by specific fugetaxis-inducing factors to which only mature cells respond.

Original languageEnglish (US)
Pages (from-to)1101-1110
Number of pages10
JournalJournal of Clinical Investigation
Volume109
Issue number8
DOIs
StatePublished - 2002
Externally publishedYes

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Emigration and Immigration
Thymocytes
Thymus Gland
T-Lymphocytes
Chemokine CXCL12
Chemotactic Factors
Cell Movement
Blood Vessels
Blood Cells
Hemodynamics
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Poznansky, M. C., Olszak, I. T., Evans, R. H., Wang, Z. Z., Foxall, R. B., Olson, D. P., ... Scadden, D. T. (2002). Thymocyte emigration is mediated by active movement away from stroma-derived factors. Journal of Clinical Investigation, 109(8), 1101-1110. https://doi.org/10.1172/JCI200213853

Thymocyte emigration is mediated by active movement away from stroma-derived factors. / Poznansky, Mark C.; Olszak, Ivona T.; Evans, Richard H.; Wang, Zack Z.; Foxall, Russell B.; Olson, Douglas P.; Weibrecht, Kathryn; Luster, Andrew D.; Scadden, David T.

In: Journal of Clinical Investigation, Vol. 109, No. 8, 2002, p. 1101-1110.

Research output: Contribution to journalArticle

Poznansky, MC, Olszak, IT, Evans, RH, Wang, ZZ, Foxall, RB, Olson, DP, Weibrecht, K, Luster, AD & Scadden, DT 2002, 'Thymocyte emigration is mediated by active movement away from stroma-derived factors', Journal of Clinical Investigation, vol. 109, no. 8, pp. 1101-1110. https://doi.org/10.1172/JCI200213853
Poznansky, Mark C. ; Olszak, Ivona T. ; Evans, Richard H. ; Wang, Zack Z. ; Foxall, Russell B. ; Olson, Douglas P. ; Weibrecht, Kathryn ; Luster, Andrew D. ; Scadden, David T. / Thymocyte emigration is mediated by active movement away from stroma-derived factors. In: Journal of Clinical Investigation. 2002 ; Vol. 109, No. 8. pp. 1101-1110.
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