Abstract
The growth and differentiation of megakaryocytes are regulated by thrombopoietin (TPO), a recently characterized cytokine which exerts its effects via a member of the hematopoietin receptor superfamily, c-Mpl. Since many cytokines which bind hematopoietin receptors activate the STAT family of transcription factors, we investigated whether STAT proteins were activated by TPO. TPO induced the formation of a DNA-binding complex recognizing a known STAT binding sequence. STAT5 was a major component of this DNA-binding complex, and STAT5 was tyrosine phosphorylated in response to TPO. Additionally, TPO-induced the tyrosine phosphorylation and DNA-binding activity of STAT3. Together with the recent demonstration of JAK2 activation in response to TPO, the data presented here define a rapid signaling pathway likely to be important in TPO-induced gene regulation.
Original language | English (US) |
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Pages (from-to) | 63-68 |
Number of pages | 6 |
Journal | FEBS Letters |
Volume | 370 |
Issue number | 1-2 |
DOIs | |
State | Published - Aug 14 1995 |
Externally published | Yes |
Keywords
- Signal transducers and activators of transcription (STAT)
- Signal transduction
- Thrombopoietin
- Tyrosine phosphorylation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology