Three serologic tests for candidiasis. Diagnostic value in distinguishing deep or disseminated infection from superficial infection or colonization

S. A. Harding, G. R. Sandford, W. G. Merz

Research output: Contribution to journalArticle

Abstract

One hundred fifty one sera from 100 hospitalized patients with positive cultures for yeasts were assayed using whole cell agglutination (Aggl.), agar gel diffusion (AGD), and counterimmunoelectrophoresis (CEP) to determine the relative diagnostic values of three serologic tests for anti Candida antibodies. Serial samples were obtained from 29 patients. Tests were read blindly; correlations of the three test results with culture results and clinical findings were determined only after all data had been accumulated. Thirty five of 100 patients had Aggl. titers of 1:160 or greater, although 13/35 had no evidence of deep or disseminated disease. Twenty four of 100 patients had clinical or autopsy evidence of deep or disseminated candidiasis; 22/24 had Aggl. titers of 1:160 or greater. Twenty of the 24 patients were CEP positive, wereas 18/24 were AGD positive. In five patients CEP became positive earlier (10-21 days) than AGD. Three patients had false positive precipitin tests, two by both CEP and AGD and the third by CEP only. In this population, a positive CEP and a positive AGD test showed good correlation with deep or disseminated candidiasis, whereas a negative Aggl. test showed the best correlation for excluding deep or systemic candidiasis.

Original languageEnglish (US)
Pages (from-to)1001-1009
Number of pages9
JournalAmerican journal of clinical pathology
Volume65
Issue number6
DOIs
StatePublished - Jan 1 1976

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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