Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

William C. Earnshaw, Barbara R Migeon

Research output: Contribution to journalArticle

Abstract

We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

Original languageEnglish (US)
Pages (from-to)290-296
Number of pages7
JournalChromosoma
Volume92
Issue number4
DOIs
StatePublished - Aug 1985

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Centromere
Chromosomes
Proteins
Chromosome Banding
Human Chromosomes
X Chromosome
Indirect Fluorescent Antibody Technique
Antigens

ASJC Scopus subject areas

  • Genetics

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Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome. / Earnshaw, William C.; Migeon, Barbara R.

In: Chromosoma, Vol. 92, No. 4, 08.1985, p. 290-296.

Research output: Contribution to journalArticle

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