Three related centromere proteins are absent from the inactive centromere of a stable isodicentric chromosome

William C. Earnshaw, Barbara R. Migeon

Research output: Contribution to journalArticle


We developed an aqueous spreading procedure that permits simultaneous analysis of human chromosomes by Q-banding and indirect immunofluorescence. Using this methodology and anticentromere antibodies from an autoimmune patient we compared the active and inactive centromeres of an isodicentric X chromosome. We show that a family of structurally related human centromere proteins (CENP-A, CENP-B, and CENP-C) is detectable only at the active centromere. These antigens therefore may be regarded both as morphological and functional markers for active centromeres.

Original languageEnglish (US)
Pages (from-to)290-296
Number of pages7
Issue number4
StatePublished - Aug 1 1985


ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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