Three dimensional structure of the pFc′ fragment of guinea pig IgG1

R. P. Phizackerley, B. C. Wishner, S. H. Bryant, Mario L Amzel, J. A. Lopez de Castro, R. J. Poljak

Research output: Contribution to journalArticle

Abstract

The three-dimensional structure of the pFc′ fragment of guinea pig IgGl, a dimer of CH3 homology sub-units, has been determined to 3.125 Å nominal resolution by crystallographic analysis. The crystal structure was solved by molecular replacement methods using as a probe the refined structure of a human IgG1 CH1 homology sub-unit. The path of the polypeptide chain has been traced from Gly341 to Ile441. No continuous electron density was found for the N-terminal segment before Gly341. The majority of the amino acid side chains were located in the electron density map. The overall tertiary structure and many features of secondary structure found in other constant region homology sub-units of immunoglobulins are preserved in pFc′. The high homology in amino acid sequence among CH3 regions of different immunoglobulins can be partly explained on the basis of the three-dimensional structure; residues which are involved in contacts essential to the tertiary and quaternary structure of the molecule are conserved or conservatively replaced. Amino acid sequence differences in the CH3 regions of different sub-classes of IgG are analyzed with respect to their position in the three-dimensional structure and the effector function properties of the different sub-classes.

Original languageEnglish (US)
Pages (from-to)841-850
Number of pages10
JournalMolecular Immunology
Volume16
Issue number11
DOIs
StatePublished - 1979

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Immunoglobulins
Guinea Pigs
Immunoglobulin G
Electrons
Amino Acid Sequence Homology
Amino Acid Sequence
Amino Acids
Peptides

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Phizackerley, R. P., Wishner, B. C., Bryant, S. H., Amzel, M. L., Lopez de Castro, J. A., & Poljak, R. J. (1979). Three dimensional structure of the pFc′ fragment of guinea pig IgG1. Molecular Immunology, 16(11), 841-850. https://doi.org/10.1016/0161-5890(79)90083-X

Three dimensional structure of the pFc′ fragment of guinea pig IgG1. / Phizackerley, R. P.; Wishner, B. C.; Bryant, S. H.; Amzel, Mario L; Lopez de Castro, J. A.; Poljak, R. J.

In: Molecular Immunology, Vol. 16, No. 11, 1979, p. 841-850.

Research output: Contribution to journalArticle

Phizackerley, RP, Wishner, BC, Bryant, SH, Amzel, ML, Lopez de Castro, JA & Poljak, RJ 1979, 'Three dimensional structure of the pFc′ fragment of guinea pig IgG1', Molecular Immunology, vol. 16, no. 11, pp. 841-850. https://doi.org/10.1016/0161-5890(79)90083-X
Phizackerley RP, Wishner BC, Bryant SH, Amzel ML, Lopez de Castro JA, Poljak RJ. Three dimensional structure of the pFc′ fragment of guinea pig IgG1. Molecular Immunology. 1979;16(11):841-850. https://doi.org/10.1016/0161-5890(79)90083-X
Phizackerley, R. P. ; Wishner, B. C. ; Bryant, S. H. ; Amzel, Mario L ; Lopez de Castro, J. A. ; Poljak, R. J. / Three dimensional structure of the pFc′ fragment of guinea pig IgG1. In: Molecular Immunology. 1979 ; Vol. 16, No. 11. pp. 841-850.
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