Abstract
Bdellovibrio bacteriovorus cells are small deltaproteobacterial cells that feed on other gram-negative bacteria, including human pathogens. Using cryo-electron tomography, we demonstrated that B. bacteriovorus cells are capable of substantial flexibility and local deformation of the outer and inner membranes without loss of cell integrity. These shape changes can occur in less than 2 min, and analysis of the internal architecture of highly bent cells showed that the overall distribution of molecular machines and the nucleoid is similar to that in moderately bent cells. B. bacteriovorus cells appear to contain an extensive internal network of short and long filamentous structures. We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2588-2596 |
| Number of pages | 9 |
| Journal | Journal of Bacteriology |
| Volume | 190 |
| Issue number | 7 |
| DOIs | |
| State | Published - Apr 2008 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography. / Borgnia, Mario J.; Subramaniam, Sriram; Milne, Jacqueline L S.
In: Journal of Bacteriology, Vol. 190, No. 7, 04.2008, p. 2588-2596.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography
AU - Borgnia, Mario J.
AU - Subramaniam, Sriram
AU - Milne, Jacqueline L S
PY - 2008/4
Y1 - 2008/4
N2 - Bdellovibrio bacteriovorus cells are small deltaproteobacterial cells that feed on other gram-negative bacteria, including human pathogens. Using cryo-electron tomography, we demonstrated that B. bacteriovorus cells are capable of substantial flexibility and local deformation of the outer and inner membranes without loss of cell integrity. These shape changes can occur in less than 2 min, and analysis of the internal architecture of highly bent cells showed that the overall distribution of molecular machines and the nucleoid is similar to that in moderately bent cells. B. bacteriovorus cells appear to contain an extensive internal network of short and long filamentous structures. We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey.
AB - Bdellovibrio bacteriovorus cells are small deltaproteobacterial cells that feed on other gram-negative bacteria, including human pathogens. Using cryo-electron tomography, we demonstrated that B. bacteriovorus cells are capable of substantial flexibility and local deformation of the outer and inner membranes without loss of cell integrity. These shape changes can occur in less than 2 min, and analysis of the internal architecture of highly bent cells showed that the overall distribution of molecular machines and the nucleoid is similar to that in moderately bent cells. B. bacteriovorus cells appear to contain an extensive internal network of short and long filamentous structures. We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey.
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UR - http://www.scopus.com/inward/citedby.url?scp=41549137007&partnerID=8YFLogxK
U2 - 10.1128/JB.01538-07
DO - 10.1128/JB.01538-07
M3 - Article
C2 - 18203829
AN - SCOPUS:41549137007
VL - 190
SP - 2588
EP - 2596
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 7
ER -