Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography

Mario J. Borgnia, Sriram Subramaniam, Jacqueline L S Milne

Research output: Contribution to journalArticle

Abstract

Bdellovibrio bacteriovorus cells are small deltaproteobacterial cells that feed on other gram-negative bacteria, including human pathogens. Using cryo-electron tomography, we demonstrated that B. bacteriovorus cells are capable of substantial flexibility and local deformation of the outer and inner membranes without loss of cell integrity. These shape changes can occur in less than 2 min, and analysis of the internal architecture of highly bent cells showed that the overall distribution of molecular machines and the nucleoid is similar to that in moderately bent cells. B. bacteriovorus cells appear to contain an extensive internal network of short and long filamentous structures. We propose that rearrangements of these structures, in combination with the unique properties of the cell envelope, may underlie the remarkable ability of B. bacteriovorus cells to find and enter bacterial prey.

Original languageEnglish (US)
Pages (from-to)2588-2596
Number of pages9
JournalJournal of Bacteriology
Volume190
Issue number7
DOIs
StatePublished - Apr 2008
Externally publishedYes

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Electron Microscope Tomography
Three-Dimensional Imaging
Bdellovibrio bacteriovorus
Gram-Negative Bacteria

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography. / Borgnia, Mario J.; Subramaniam, Sriram; Milne, Jacqueline L S.

In: Journal of Bacteriology, Vol. 190, No. 7, 04.2008, p. 2588-2596.

Research output: Contribution to journalArticle

Borgnia, Mario J. ; Subramaniam, Sriram ; Milne, Jacqueline L S. / Three-dimensional imaging of the highly bent architecture of Bdellovibrio bacteriovorus by using cryo-electron tomography. In: Journal of Bacteriology. 2008 ; Vol. 190, No. 7. pp. 2588-2596.
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