Abstract
By the systemic administration of diethyldithiocarbamate and iron into the rat, nitric oxide radicals produced in the brain during ischemia-hypoxia were trapped. The right hemisphere of the brain was then removed and frozen with liquid nitrogen. With use of recently developed electron paramagnetic resonance imaging instrumentation and techniques, three-dimensional imaging of the production of the nitric oxide radicals in several brains was performed. The results suggest that nitric oxide radicals were produced and trapped in the areas that are known to have high nitric oxide synthase activity, such as cortex, hippocampus, hypothalamus, amygdala, and substantia nigra. In this ischemia-hypoxia model, which did not interrupt the posterior circulation, the production and trapping of nitric oxide in the cerebellum were ~30% of those in the cerebrum.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 899-903 |
| Number of pages | 5 |
| Journal | Journal of Cerebral Blood Flow and Metabolism |
| Volume | 15 |
| Issue number | 6 |
| State | Published - 1995 |
| Externally published | Yes |
Fingerprint
Keywords
- Diethyldithiocarbamate
- Electron paramagnetic resonance imaging
- hypoxia
- Ischemia
- Nitric oxide
- Spin trapping
- Three-dimensional imaging
ASJC Scopus subject areas
- Endocrinology
- Endocrinology, Diabetes and Metabolism
- Neuroscience(all)
Cite this
Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia. / Kuppusamy, P.; Ohnishi, S. T.; Numagami, Y.; Ohnishi, T.; Zweier, J. L.
In: Journal of Cerebral Blood Flow and Metabolism, Vol. 15, No. 6, 1995, p. 899-903.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia
AU - Kuppusamy, P.
AU - Ohnishi, S. T.
AU - Numagami, Y.
AU - Ohnishi, T.
AU - Zweier, J. L.
PY - 1995
Y1 - 1995
N2 - By the systemic administration of diethyldithiocarbamate and iron into the rat, nitric oxide radicals produced in the brain during ischemia-hypoxia were trapped. The right hemisphere of the brain was then removed and frozen with liquid nitrogen. With use of recently developed electron paramagnetic resonance imaging instrumentation and techniques, three-dimensional imaging of the production of the nitric oxide radicals in several brains was performed. The results suggest that nitric oxide radicals were produced and trapped in the areas that are known to have high nitric oxide synthase activity, such as cortex, hippocampus, hypothalamus, amygdala, and substantia nigra. In this ischemia-hypoxia model, which did not interrupt the posterior circulation, the production and trapping of nitric oxide in the cerebellum were ~30% of those in the cerebrum.
AB - By the systemic administration of diethyldithiocarbamate and iron into the rat, nitric oxide radicals produced in the brain during ischemia-hypoxia were trapped. The right hemisphere of the brain was then removed and frozen with liquid nitrogen. With use of recently developed electron paramagnetic resonance imaging instrumentation and techniques, three-dimensional imaging of the production of the nitric oxide radicals in several brains was performed. The results suggest that nitric oxide radicals were produced and trapped in the areas that are known to have high nitric oxide synthase activity, such as cortex, hippocampus, hypothalamus, amygdala, and substantia nigra. In this ischemia-hypoxia model, which did not interrupt the posterior circulation, the production and trapping of nitric oxide in the cerebellum were ~30% of those in the cerebrum.
KW - Diethyldithiocarbamate
KW - Electron paramagnetic resonance imaging
KW - hypoxia
KW - Ischemia
KW - Nitric oxide
KW - Spin trapping
KW - Three-dimensional imaging
UR - http://www.scopus.com/inward/record.url?scp=0028875770&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028875770&partnerID=8YFLogxK
M3 - Article
C2 - 7593349
AN - SCOPUS:0028875770
VL - 15
SP - 899
EP - 903
JO - Journal of Cerebral Blood Flow and Metabolism
JF - Journal of Cerebral Blood Flow and Metabolism
SN - 0271-678X
IS - 6
ER -