Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia

P. Kuppusamy, S. T. Ohnishi, Y. Numagami, T. Ohnishi, J. L. Zweier

Research output: Contribution to journalArticle

Abstract

By the systemic administration of diethyldithiocarbamate and iron into the rat, nitric oxide radicals produced in the brain during ischemia-hypoxia were trapped. The right hemisphere of the brain was then removed and frozen with liquid nitrogen. With use of recently developed electron paramagnetic resonance imaging instrumentation and techniques, three-dimensional imaging of the production of the nitric oxide radicals in several brains was performed. The results suggest that nitric oxide radicals were produced and trapped in the areas that are known to have high nitric oxide synthase activity, such as cortex, hippocampus, hypothalamus, amygdala, and substantia nigra. In this ischemia-hypoxia model, which did not interrupt the posterior circulation, the production and trapping of nitric oxide in the cerebellum were ~30% of those in the cerebrum.

Original languageEnglish (US)
Pages (from-to)899-903
Number of pages5
JournalJournal of Cerebral Blood Flow and Metabolism
Volume15
Issue number6
StatePublished - 1995
Externally publishedYes

Fingerprint

Three-Dimensional Imaging
Nitric Oxide
Ischemia
Brain
Ditiocarb
Brain Hypoxia-Ischemia
Electron Spin Resonance Spectroscopy
Cerebrum
Substantia Nigra
Amygdala
Nitric Oxide Synthase
Cerebellum
Hypothalamus
Hippocampus
Nitrogen
Iron
Hypoxia

Keywords

  • Diethyldithiocarbamate
  • Electron paramagnetic resonance imaging
  • hypoxia
  • Ischemia
  • Nitric oxide
  • Spin trapping
  • Three-dimensional imaging

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Neuroscience(all)

Cite this

Kuppusamy, P., Ohnishi, S. T., Numagami, Y., Ohnishi, T., & Zweier, J. L. (1995). Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia. Journal of Cerebral Blood Flow and Metabolism, 15(6), 899-903.

Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia. / Kuppusamy, P.; Ohnishi, S. T.; Numagami, Y.; Ohnishi, T.; Zweier, J. L.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 15, No. 6, 1995, p. 899-903.

Research output: Contribution to journalArticle

Kuppusamy, P, Ohnishi, ST, Numagami, Y, Ohnishi, T & Zweier, JL 1995, 'Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia', Journal of Cerebral Blood Flow and Metabolism, vol. 15, no. 6, pp. 899-903.
Kuppusamy, P. ; Ohnishi, S. T. ; Numagami, Y. ; Ohnishi, T. ; Zweier, J. L. / Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemia-hypoxia. In: Journal of Cerebral Blood Flow and Metabolism. 1995 ; Vol. 15, No. 6. pp. 899-903.
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