Three dimension optical imaging in a high throughput layered expression system

Reut Cohen, Gallya Gannot, Moshe Ban David, Nir Sochen, Israel Gannot

Research output: Contribution to journalArticlepeer-review

Abstract

Layered peptide array (LPA) system enables multiplex screening of biomarkers [1-3]. One of the main problems of the LPA system is the screening of the layered-membranes stack. Nowadays, each membrane is imaged separately using conventional fluorescent imaging. This process is time consuming and requires extensive manual interaction. This paper describes a general solution for optical imaging of a layered grid medium using photogrammetric methods. The suggested method enables visualization of the LPA membranes stack by using only two images of the stack. This study is a proof of concept of the suggested solution using MATLAB simulation and phantom experiments.

Original languageEnglish (US)
Pages (from-to)19822-19835
Number of pages14
JournalOptics Express
Volume19
Issue number21
DOIs
StatePublished - Oct 10 2011
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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