TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
|Original language||English (US)|
|Pages (from-to)||Appendix 3D|
|Journal||Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]|
|State||Published - May 2001|
ASJC Scopus subject areas
- Organic Chemistry