Thin-layer chromatography.

Research output: Contribution to journalArticle

Abstract

TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.

Original languageEnglish (US)
Pages (from-to)Appendix 3D
JournalCurrent protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.]
VolumeAppendix 3
StatePublished - May 2001
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry

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