Abstract
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
| Original language | English (US) |
|---|---|
| Journal | Current Protocols in Nucleic Acid Chemistry |
| Volume | Appendix 3 |
| State | Published - May 2001 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Medicine(all)
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Thin-layer chromatography. / Meyers, Caren L.
In: Current Protocols in Nucleic Acid Chemistry, Vol. Appendix 3, 05.2001.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Thin-layer chromatography.
AU - Meyers, Caren L
PY - 2001/5
Y1 - 2001/5
N2 - TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
AB - TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
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M3 - Article
VL - Appendix 3
JO - Current Protocols in Nucleic Acid Chemistry
JF - Current Protocols in Nucleic Acid Chemistry
SN - 1934-9270
ER -