Abstract
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
| Original language | English (US) |
|---|---|
| Pages (from-to) | Appendix 3D |
| Journal | Current protocols in nucleic acid chemistry / edited by Serge L. Beaucage ... [et al.] |
| Volume | Appendix 3 |
| State | Published - May 2001 |
| Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry