Thin-layer chromatography

Research output: Contribution to journalArticle

Abstract

TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.

Original languageEnglish (US)
JournalCurrent Protocols in Nucleic Acid Chemistry
Issue numberSUPPL. 34
DOIs
StatePublished - 2008

Fingerprint

Thin layer chromatography
Column chromatography
Thin Layer Chromatography
Chromatography
Metrorrhagia
Nucleic Acids
Chemical reactions
Assays

Keywords

  • Nucleosides
  • Nucleotides
  • Separation
  • Thin-layer chromatography
  • TLC

ASJC Scopus subject areas

  • Biochemistry
  • Organic Chemistry
  • Medicine(all)

Cite this

Thin-layer chromatography. / Meyers, Caren L; Meyers, David J.

In: Current Protocols in Nucleic Acid Chemistry, No. SUPPL. 34, 2008.

Research output: Contribution to journalArticle

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