Abstract
TLC is used extensively in nucleic acid chemistry to monitor the progress of chemical reactions, to assay fractions collected from a larger chromatographic separation (e.g., column chromatography), and to determine optimal conditions prior to column chromatography. This unit describes methods for spotting test compounds onto a TLC plate, developing the plate in a suitable solvent system, visualizing the results, and calculating the retention factor (Rf). Candidate compounds can be co-spotted for identification without relying on Rf values.
| Original language | English (US) |
|---|---|
| Pages (from-to) | A.3D.1-A.3D.13 |
| Journal | Current Protocols in Nucleic Acid Chemistry |
| Issue number | SUPPL. 34 |
| DOIs | |
| State | Published - 2008 |
Keywords
- Nucleosides
- Nucleotides
- Separation
- TLC
- Thin-layer chromatography
ASJC Scopus subject areas
- Biochemistry
- Organic Chemistry