Thin-layer chromatographic determination of urinary excretion of lactulose, simplified and applied to cystic fibrosis patients

J. A. Flick, R. L. Schnaar, J. A. Perman

Research output: Contribution to journalArticle

Abstract

Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanolborate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detected with high reproducibility and without interference by urinary chromogens. After oral administration, the urinary excretion of lactulose in 8 h equaled 2.33 (SD 1.86)% in 15 patients with cystic fibrosis, as compared with 0.13 (SD 0.12)% in 16 healthy subjects (P <0.001).

Original languageEnglish (US)
Pages (from-to)1211-1212
Number of pages2
JournalClinical chemistry
Volume33
Issue number7
DOIs
StatePublished - 1987

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, medical

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