Abstract
Urinary excretion of orally administered lactulose is used as an index of intestinal permeability. We have developed a simple thin-layer chromatographic technique for measuring lactulose in urine, using silica gel 60 plates and a propanolborate solvent system. Lactulose concentrations as low as 62.5 mg/L can be detected with high reproducibility and without interference by urinary chromogens. After oral administration, the urinary excretion of lactulose in 8 h equaled 2.33 (SD 1.86)% in 15 patients with cystic fibrosis, as compared with 0.13 (SD 0.12)% in 16 healthy subjects (P <0.001).
Original language | English (US) |
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Pages (from-to) | 1211-1212 |
Number of pages | 2 |
Journal | Clinical chemistry |
Volume | 33 |
Issue number | 7 |
DOIs | |
State | Published - 1987 |
ASJC Scopus subject areas
- Clinical Biochemistry
- Biochemistry, medical