Thermotropic Behavior of Monoglucocerebroside-Dipalmitoylphosphatidylcholine Multilamellar Liposomes

Maria C. Correa-Freire, Ernesto Freire, Yechezkel Barenholz, Rodney L. Biltonen, Thomas E. Thompson

Research output: Contribution to journalArticle

Abstract

The thermotropic behavior of multilamellar liposomes prepared from mixtures of glucocerebroside and dipalmitoylphosphatidylcholine has been studied by high-sensitivity scanning calorimetrv. It is shown that glucocerebroside has a marked effect on the gel-liquid crystalline transition of dipalmitoylphosphatidylcholine. The pretransition seen in pure samples of dipalmitoylphosphatidylcholine is undetectable at small mole fractions of glucocerebrosides (<10%). The main transition is shifted to higher temperatures and becomes broader and less cooperative in the presence of glucocerebroside. The enthalpy change of the main transition decreases with increasing the glucocerebroside content. However, this decrease is not linear with the glucocerebroside/phospholipid mole ratio. Glucocerebroside itself does not show a separate transition in the temperature range of these studies (10-75 °C). The origin of these effects and their dependence on the glucocerebroside content suggest that the in-plane distribution of glucocerebroside molecules is affected by the physical state of the lipid bilayer and by the glucocerebroside/phospholipid mole ratio.

Original languageEnglish (US)
Pages (from-to)442-445
Number of pages4
JournalBiochemistry
Volume18
Issue number3
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Biochemistry

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