Thermotropic Behavior of Aqueous Dispersions of Glucosylceramide-Dipalmitoylphosphatidylcholine Mixtures

Yechezkel Barenholz; T. E. Thompson; Ernesto Freire; Maria C. Correa-Freire; Diana Bach; Israel R. Miller

doi:10.1021/bi00283a029

Thermotropic Behavior of Aqueous Dispersions of Glucosylceramide-Dipalmitoylphosphatidylcholine Mixtures

Yechezkel Barenholz, T. E. Thompson, Ernesto Freire, Maria C. Correa-Freire, Diana Bach, Israel R. Miller

Krieger School of Arts and Sciences

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Differential scanning calorimetry has been carried out on aqueous dispersions of multilamellar liposomes formed from mixtures of glucosylceramide and dipalmitoylphosphatidylcholine. The phase diagram constructed from data obtained from dispersions in excess water in the cooling mode is simpler than the diagram obtained from data obtained in the heating mode. The essentially horizontal portion of the solidus curve up to 60% glucosylceramide indicates lateral phase separation in the gel phase. The existence of a metastable region of glucosylceramide seen in the heating scans at system concentrations greater than 90% of this component is reminiscent of the complex thermal behavior of pure glucosylceramide dispersions in excess water.

Original language	English (US)
Pages (from-to)	3497-3501
Number of pages	5
Journal	Biochemistry
Volume	22
Issue number	14
DOIs	https://doi.org/10.1021/bi00283a029
State	Published - 1983

ASJC Scopus subject areas

Biochemistry

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title = "Thermotropic Behavior of Aqueous Dispersions of Glucosylceramide-Dipalmitoylphosphatidylcholine Mixtures",

abstract = "Differential scanning calorimetry has been carried out on aqueous dispersions of multilamellar liposomes formed from mixtures of glucosylceramide and dipalmitoylphosphatidylcholine. The phase diagram constructed from data obtained from dispersions in excess water in the cooling mode is simpler than the diagram obtained from data obtained in the heating mode. The essentially horizontal portion of the solidus curve up to 60% glucosylceramide indicates lateral phase separation in the gel phase. The existence of a metastable region of glucosylceramide seen in the heating scans at system concentrations greater than 90% of this component is reminiscent of the complex thermal behavior of pure glucosylceramide dispersions in excess water.",

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T1 - Thermotropic Behavior of Aqueous Dispersions of Glucosylceramide-Dipalmitoylphosphatidylcholine Mixtures

AU - Barenholz, Yechezkel

AU - Thompson, T. E.

AU - Freire, Ernesto

AU - Correa-Freire, Maria C.

AU - Bach, Diana

AU - Miller, Israel R.

PY - 1983

Y1 - 1983

N2 - Differential scanning calorimetry has been carried out on aqueous dispersions of multilamellar liposomes formed from mixtures of glucosylceramide and dipalmitoylphosphatidylcholine. The phase diagram constructed from data obtained from dispersions in excess water in the cooling mode is simpler than the diagram obtained from data obtained in the heating mode. The essentially horizontal portion of the solidus curve up to 60% glucosylceramide indicates lateral phase separation in the gel phase. The existence of a metastable region of glucosylceramide seen in the heating scans at system concentrations greater than 90% of this component is reminiscent of the complex thermal behavior of pure glucosylceramide dispersions in excess water.

AB - Differential scanning calorimetry has been carried out on aqueous dispersions of multilamellar liposomes formed from mixtures of glucosylceramide and dipalmitoylphosphatidylcholine. The phase diagram constructed from data obtained from dispersions in excess water in the cooling mode is simpler than the diagram obtained from data obtained in the heating mode. The essentially horizontal portion of the solidus curve up to 60% glucosylceramide indicates lateral phase separation in the gel phase. The existence of a metastable region of glucosylceramide seen in the heating scans at system concentrations greater than 90% of this component is reminiscent of the complex thermal behavior of pure glucosylceramide dispersions in excess water.

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Thermotropic Behavior of Aqueous Dispersions of Glucosylceramide-Dipalmitoylphosphatidylcholine Mixtures

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