Thermotolerance of human myometrium: Implications for minimally invasive uterine therapies

Aaron C. Thomas, Brian T. Grisez, Kathleen McMillan, Nicholas Chill, Tyler P. Harclerode, Rebecca Radabaugh, Ryan M. Jones, James E. Coad

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Endometrial ablation has gained significant clinical acceptance over the last decade as a minimally invasive treatment for abnormal uterine bleeding. To improve upon current thermal injury modeling, it is important to better characterize the myometrium's thermotolerance. The extent of myometrial thermal injury was determined across a spectrum of thermal histories/doses (time-temperature combinations). Fresh extirpated human myometrium was obtained from 13 subjects who underwent a previous scheduled benign hysterectomy. Within two hours of hysterectomy, the unfixed myometrium was treated in a stabilized saline bath with temperatures ranging from 45-70°C and time intervals from 30- 150 seconds. The time-temperature combinations were selected to simulate treatment times under 2.5 minutes. A total of six such thermal matrices, each comprised of 45 time-temperature combinations, were prepared for evaluation. The treated myometrium was cryosectioned for nitro blue tetrazolium (NBT) staining to assess for thermal respiratory enzyme inactivation. Image analysis was subsequently used to quantitatively assess the stained myometrium's capacity to metabolize the tetrazolium at each time-temperature combination. This colorimetric data was then used as marker of cellular viability and determine survival parameters with implications for developing minimally invasive uterine therapies.

Original languageEnglish (US)
Title of host publicationEnergy-Based Treatment of Tissue and Assessment VII
DOIs
StatePublished - Jun 10 2013
Externally publishedYes
Event2013 SPIE Conference: Energy-Based Treatment of Tissue and Assessment VII - San Francisco, CA, United States
Duration: Feb 3 2013Feb 4 2013

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8584
ISSN (Print)1605-7422

Other

Other2013 SPIE Conference: Energy-Based Treatment of Tissue and Assessment VII
CountryUnited States
CitySan Francisco, CA
Period2/3/132/4/13

Keywords

  • Endometrial ablation
  • histology
  • menorrhagia
  • nitro blue tetrazolium staining
  • quantitative image analysis
  • thermal modeling
  • thermal therapy
  • tissue ablation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Thomas, A. C., Grisez, B. T., McMillan, K., Chill, N., Harclerode, T. P., Radabaugh, R., Jones, R. M., & Coad, J. E. (2013). Thermotolerance of human myometrium: Implications for minimally invasive uterine therapies. In Energy-Based Treatment of Tissue and Assessment VII [858406] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 8584). https://doi.org/10.1117/12.2006867