Thermodynamics of Intersubunit Interactions in Cholera Toxin upon Binding to the Oligosaccharide Portion of Its Cell Surface Receptor, Ganglioside GM1

Arne Schön, Ernesto Freire, Arne Schön

Research output: Contribution to journalArticle

Abstract

The binding and the energetics of the interaction of cholera toxin with the oligosaccharide portion of ganglioside GM1 (oligo-GM1), the toxin cell surface receptor, have been studied by high-sensitivity isothermal titration calorimetry and differential scanning calorimetry. Previously, we have shown that the association of cholera toxin to ganglioside GM1 enhances the cooperative interactions between subunits in the B-subunit pentamer [Goins, B., & Freire, E. (1988) Biochemistry 27, 2046-2052], New experiments presented in this paper reveal that the oligosaccharide portion of the receptor is by itself able to enhance the intersubunit cooperative interactions within the B pentamer. This effect is seen in the protein unfolding transition as a shift from independent unfolding of the B promoters toward a cooperative unfolding. To identify the origin of this effect, the binding of cholera toxin to oligo-GM1 has been measured calorimetrically under isothermal conditions. The binding curve at 37 °C is sigmoidal, indicating cooperative binding. The binding data can be described in terms of a nearest-neighbor cooperative interaction binding model. In terms of this model, the association of a oligo-GM1 molecule to a B protomer affects the association to adjacent B promoters within the pentameric ring. The measured intrinsic binding enthalpy per protomer is -22 kcal/mol and the cooperative interaction enthalpy -11 kcal/mol. The intrinsic binding constant determined calorimetrically is 1.05 × 106 M-1 at 37 °C and the cooperative Gibbs free energy equal to -850 cal/mol. These studies provide the first direct thermodynamic description of the cooperative binding of a protein to its cell surface receptor and have allowed us to place energetics constraints on the putative changes in protein conformation triggered by the intrinsic receptor binding process.

Original languageEnglish (US)
Pages (from-to)5019-5024
Number of pages6
JournalBiochemistry
Volume28
Issue number12
DOIs
StatePublished - Jun 1 1989

ASJC Scopus subject areas

  • Biochemistry

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