Thermal stability of an oral killed-cholera-whole-cell vaccine containing recombinant B-subunit of cholera toxin

Z. Uddin Ahmed, M. Mainul Hoque, A. S M Hamidur Rahman, R. Bardley Sack

Research output: Contribution to journalArticle

Abstract

An oral killed cholera vaccine containing 1x1011 cells of Vibrio cholerae O1 (heat- or formalin-killed) representing the Ogawa and Inaba biotypes and containing 1 mg of B-subunit of cholera toxin (CTB) produced by recombinant DNA technology (the WC/rCTB vaccine) was subjected to temperatures of 4 C, 30 C or 42 C for up to 6 months time. Lipopolysaccharide antigen (LPS) and CTB content of the vaccine samples determined at various times remained unchanged during the study except for the CTB component which decreased by about 50% after 6 months of storage at 42 C. Immunogenicity determined by immunization of rabbits with the vaccine in Freund's complete adjuvant and measuring anti-LPS and anti-CTB antibody titers in the serum by an ELISA was also found to be unaltered. Lyophilization of the vaccine and storage at room temperature for 7 days also did not have any adverse effect on antigen content or immunogenicity as tested above. There was up to one log reduction in serum antibody titers after immunization without using any adjuvant or using Freund's incomplete adjuvant, and up to two logs following oral immunization. Immunization by oral feeding of the vaccine followed by RITARD challenge with a virulent V. cholerae O1 strain showed evidence of protection against severe or lethal diarrhea. The results suggest that the vaccine retains its antigen content and ability to induce antibodies unchanged when maintained at elevated temperatures for relatively long periods of time.

Original languageEnglish (US)
Pages (from-to)837-842
Number of pages6
JournalMicrobiology and Immunology
Volume38
Issue number11
StatePublished - 1994
Externally publishedYes

Fingerprint

Synthetic Vaccines
Cholera
Cholera Toxin
Vaccines
Hot Temperature
Immunization
Vibrio cholerae O1
Antigens
Temperature
Lipopolysaccharides
Cholera Vaccines
Inactivated Vaccines
Freeze Drying
Recombinant DNA
Freund's Adjuvant
Antibodies
Serum
Formaldehyde
Anti-Idiotypic Antibodies
Diarrhea

Keywords

  • Cholera-toxin
  • Killed-vibrio
  • Vaccine

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Microbiology
  • Microbiology (medical)

Cite this

Uddin Ahmed, Z., Mainul Hoque, M., Hamidur Rahman, A. S. M., & Bardley Sack, R. (1994). Thermal stability of an oral killed-cholera-whole-cell vaccine containing recombinant B-subunit of cholera toxin. Microbiology and Immunology, 38(11), 837-842.

Thermal stability of an oral killed-cholera-whole-cell vaccine containing recombinant B-subunit of cholera toxin. / Uddin Ahmed, Z.; Mainul Hoque, M.; Hamidur Rahman, A. S M; Bardley Sack, R.

In: Microbiology and Immunology, Vol. 38, No. 11, 1994, p. 837-842.

Research output: Contribution to journalArticle

Uddin Ahmed, Z, Mainul Hoque, M, Hamidur Rahman, ASM & Bardley Sack, R 1994, 'Thermal stability of an oral killed-cholera-whole-cell vaccine containing recombinant B-subunit of cholera toxin', Microbiology and Immunology, vol. 38, no. 11, pp. 837-842.
Uddin Ahmed, Z. ; Mainul Hoque, M. ; Hamidur Rahman, A. S M ; Bardley Sack, R. / Thermal stability of an oral killed-cholera-whole-cell vaccine containing recombinant B-subunit of cholera toxin. In: Microbiology and Immunology. 1994 ; Vol. 38, No. 11. pp. 837-842.
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