Thermal latency studies in opiate-treated mice

Noam Schildhaus, Eliana Trink, Chirs Polson, Louis Detolla, Betty Mae Tyler, George Jallo, Sino Tok, Michael Guarnieri

Research output: Contribution to journalArticle

Abstract

Background: The change in the reaction time of a tail or paw exposed to a thermal stimulus is a measure of nociceptive activity in laboratory animals. Tail-flick and plantar thermal sensitivity (Hargreaves) tests are non-invasive, minimize stress, and can be used to screen animals for phenotype and drug activity. Objective: Hargreaves testing has been widely used in rats. We investigated its use to measure the activity of opiate analgesia in mice. Methods: Mice were used in thermal stimulus studies at 1-5 hours and 1-5 days to test acute and extended release preparations of buprenorphine. Results: Hargreaves testing had limited value at 1-5 hours because mice can have an obtunded response to opiate therapy. Tail-flick studies with restrained mice are not affected by the initial locomotor stimulation. Discussion: The present report describes a simple restraint system for mice. The utility of the system is demonstrated by examining the efficacy of acute and extended release buprenorphine injections in Balb/c and Swiss mice. Conclusion: Standardized tail-flick testing provides a sensitive robust method to monitor opiate activity in mice.

Original languageEnglish (US)
Pages (from-to)43-47
Number of pages5
JournalJournal of Pharmacy and Bioallied Sciences
Volume6
Issue number1
DOIs
StatePublished - 2014

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Keywords

  • Analgesia
  • buprenorphine
  • extended release
  • Hargreaves test
  • mouse
  • thermal latency test

ASJC Scopus subject areas

  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Bioengineering

Cite this

Schildhaus, N., Trink, E., Polson, C., Detolla, L., Tyler, B. M., Jallo, G., Tok, S., & Guarnieri, M. (2014). Thermal latency studies in opiate-treated mice. Journal of Pharmacy and Bioallied Sciences, 6(1), 43-47. https://doi.org/10.4103/0975-7406.124316