Thermal denaturation of type i collagen vitrified gels

Zhiyong Xia, Xiomara Calderon-Colon, Morgana Trexler, Jennifer Elisseeff, Qiongyu Guo

Research output: Contribution to journalArticlepeer-review

Abstract

The denaturation kinetics of type I collagen vitrigels synthesized under different vitrification time and temperature were analyzed by the classical Kissinger approach and the advanced model free kinetics (AMFK) using the Vyazovkin algorithm. The AMFK successfully elucidated the overall denaturation into reversible and irreversible processes. Depending on vitrification conditions, the activation energy for the irreversible process ranged from 100 to 200 kJ/mol, and the reversible enthalpy ranged from 250 to 300 kJ/mol. All of these values increased with the vitrification time and temperature, indicating that a more stable and complex structure formed with increased vitrification. The classical Kissinger method predicted the presence of a critical temperate of approximately 60 °C for the transition between reversible and irreversible processes. Scanning electron microscopy revealed the presence of fibril structures in vitrigels both before and after full denaturation; however the fibrils had became thicker and rougher after denaturation.

Original languageEnglish (US)
Pages (from-to)172-179
Number of pages8
JournalThermochimica Acta
Volume527
DOIs
StatePublished - Jan 10 2012

Keywords

  • Denaturation kinetics
  • Kissinger approach
  • Model free kinetics
  • Thermal activation energy
  • Vitrified collagen gel

ASJC Scopus subject areas

  • Instrumentation
  • Condensed Matter Physics
  • Physical and Theoretical Chemistry

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