Mycobacterium phlei hücre duvari ekstrelerinin terapötik etkileri

Translated title of the contribution: Therapeutic effects of mycobacterium phlei cell wall extracts

Baǧnu Orhan, Esra Buber, Mehmet Selcuk Keskin, Omur Celikbicak, Bekir Salih, Alpaslan Alp, Zeynep Saribaş, Abdurrahim Haluk Ozen, Naciye Leyla Acan

Research output: Contribution to journalArticle

Abstract

Aim: To investigate the cytokine-stimulating activity of several Mycobacterium phlei cell wall extracts. The bacillus Calmette-Guerin application is the gold standard treatment for some types of superfi cial bladder carcinoma. However, its usage is limited due to its harmful side effects. Intensive research has been carried out to fi nd less toxic and more potent therapeutic agents for treatment. Researchers focus on M. phlei for this purpose. Th e cell wall extract of M. phlei is suffi cient for antitumoral activity. Materials and methods: In this study, M. phlei cells were cultured and several different cell wall extracts were prepared by physicochemical procedures. Human monocytic cell lines were incubated with these extracts, and tumor necrosis factor-α and interleukin-12 were determined in the samples. Results: Various cell extracts showed tumor necrosis factor-α and interleukin-12 stimulating activity. Matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS) profi les of the samples were also obtained. Th e MALDIMS spectra of the samples showed that peptides of 3808, 9207, 11,533, 12,460, and 21,587 Da may be involved in the immunostimulation activity. Conclusion: Th is study showed that peptides obtained from mycobacteria cell wall extracts may be used to prepare new antitumoral chemicals for the treatment of superfi cial bladder carcinoma.

Original languageTurkish
Pages (from-to)193-199
Number of pages7
JournalTurkish Journal of Medical Sciences
Volume42
Issue number2
DOIs
StatePublished - Mar 1 2012

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Keywords

  • Cell wall
  • Interleukin-12
  • Maldi-ms spectra
  • Mycobacterium phlei
  • Tumor necrosis factor-α

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Orhan, B., Buber, E., Keskin, M. S., Celikbicak, O., Salih, B., Alp, A., Saribaş, Z., Ozen, A. H., & Acan, N. L. (2012). Mycobacterium phlei hücre duvari ekstrelerinin terapötik etkileri. Turkish Journal of Medical Sciences, 42(2), 193-199. https://doi.org/10.3906/sag-1010-1235