Therapeutic effects of adipose-tissue-derived mesenchymal stromal cells and their extracellular vesicles in experimental silicosis

Elga Bandeira, Helena Oliveira, Johnatas D. Silva, Rubem F.S. Menna-Barreto, Christina M. Takyia, Jung Soo Suk, Kenneth Whitaker Witwer, Michael Paulaitis, Justin S Hanes, Patricia R.M. Rocco, Marcelo M. Morales

Research output: Contribution to journalArticle

Abstract

Background: Silicosis is an occupational disease that affects workers who inhale silica particles, leading to extensive lung fibrosis and ultimately causing respiratory failure. Mesenchymal stromal cells (MSCs) have been shown to exert therapeutic effects in lung diseases and represent an alternative treatment for silicosis. Recently, it has been suggested that similar effects can be achieved by the therapeutic use of extracellular vesicles (EVs) obtained from MSCs. The aim of this study was to investigate the effects of adipose-tissue-derived MSCs (AD-MSCs) or their EVs in a model of silicosis. Methods: Silicosis was induced by intratracheal instillation of silica in C57BL/6 mice. After the onset of disease, animals received saline, AD-MSCs, or EVs, intratracheally. Results: At day 30, AD-MSCs and EVs led to a reduction in collagen fiber content, size of granuloma, and in the number of macrophages inside granuloma and in the alveolar septa. In addition, the expression levels of interleukin 1β and transforming growth factor beta in the lungs were decreased. Higher dose of EVs also reduced lung static elastance when compared with the untreated silicosis group. Conclusions: Both AD-MSCs and EVs, locally delivered, ameliorated fibrosis and inflammation, but dose-enhanced EVs yielded better therapeutic outcomes in this model of silicosis.

Original languageEnglish (US)
Article number104
JournalRespiratory Research
Volume19
Issue number1
DOIs
StatePublished - May 29 2018

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Silicosis
Therapeutic Uses
Mesenchymal Stromal Cells
Adipose Tissue
Granuloma
Silicon Dioxide
Lung
Fibrosis
Animal Diseases
Occupational Diseases
Extracellular Vesicles
Interleukin-1
Inbred C57BL Mouse
Respiratory Insufficiency
Transforming Growth Factor beta
Lung Diseases
Collagen
Macrophages
Inflammation

Keywords

  • Extracellular vesicles
  • Fibrosis
  • Inflammation
  • Mesenchymal stromal cells
  • Silicosis

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Cite this

Therapeutic effects of adipose-tissue-derived mesenchymal stromal cells and their extracellular vesicles in experimental silicosis. / Bandeira, Elga; Oliveira, Helena; Silva, Johnatas D.; Menna-Barreto, Rubem F.S.; Takyia, Christina M.; Suk, Jung Soo; Witwer, Kenneth Whitaker; Paulaitis, Michael; Hanes, Justin S; Rocco, Patricia R.M.; Morales, Marcelo M.

In: Respiratory Research, Vol. 19, No. 1, 104, 29.05.2018.

Research output: Contribution to journalArticle

Bandeira, Elga ; Oliveira, Helena ; Silva, Johnatas D. ; Menna-Barreto, Rubem F.S. ; Takyia, Christina M. ; Suk, Jung Soo ; Witwer, Kenneth Whitaker ; Paulaitis, Michael ; Hanes, Justin S ; Rocco, Patricia R.M. ; Morales, Marcelo M. / Therapeutic effects of adipose-tissue-derived mesenchymal stromal cells and their extracellular vesicles in experimental silicosis. In: Respiratory Research. 2018 ; Vol. 19, No. 1.
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AU - Suk, Jung Soo

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