Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia-reperfusion injury

Rachel J. Santora, Mihaela L. Lie, Dmitry N. Grigoryev, Omer Nasir, Frederick A. Moore, Heitham T Hassoun

Research output: Contribution to journalArticle

Abstract

Introduction: Mesenteric ischemia-reperfusion injury (IRI) leads to systemic inflammation and multiple organ failure in clinical and laboratory settings. We investigated the lung structural, functional, and genomic response to mesenteric IRI with and without regional intraischemic hypothermia (RIH) in rodents and hypothesized that RIH would protect the lung and preferentially modulate the distant organ transcriptome under these conditions. Methods: Sprague-Dawley rats underwent sham laparotomy or superior mesenteric artery occlusion (SMAO) for 60 minutes with or without RIH. Gut temperature was maintained at 15°-20°C during SMAO, and systemic normothermia (37°C) was maintained throughout the study period. At 6 or 24 hours, lung tissue was collected for (1) histology, (2) myeloperoxidase activity, (3) bronchoalveolar lavage (BAL) fluid protein concentrations, (4) lung wet/dry ratios, and (5) total RNA isolation and hybridization to Illumina's Sentrix BeadChips (>22,000 probes) for gene expression profiling. Significantly affected genes (false discovery rate

Original languageEnglish (US)
Pages (from-to)1003-1014
Number of pages12
JournalJournal of Vascular Surgery
Volume52
Issue number4
DOIs
StatePublished - Oct 2010
Externally publishedYes

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Hypothermia
Reperfusion Injury
Superior Mesenteric Artery
Lung
Multiple Organ Failure
Bronchoalveolar Lavage Fluid
Genetic Association Studies
Gene Expression Profiling
Pulmonary Edema
Transcriptome
Laparotomy
Peroxidase
Sprague Dawley Rats
Rodentia
Histology
Therapeutics
RNA
Inflammation
Temperature
Mesenteric Ischemia

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Medicine(all)

Cite this

Santora, R. J., Lie, M. L., Grigoryev, D. N., Nasir, O., Moore, F. A., & Hassoun, H. T. (2010). Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia-reperfusion injury. Journal of Vascular Surgery, 52(4), 1003-1014. https://doi.org/10.1016/j.jvs.2010.05.088

Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia-reperfusion injury. / Santora, Rachel J.; Lie, Mihaela L.; Grigoryev, Dmitry N.; Nasir, Omer; Moore, Frederick A.; Hassoun, Heitham T.

In: Journal of Vascular Surgery, Vol. 52, No. 4, 10.2010, p. 1003-1014.

Research output: Contribution to journalArticle

Santora, RJ, Lie, ML, Grigoryev, DN, Nasir, O, Moore, FA & Hassoun, HT 2010, 'Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia-reperfusion injury', Journal of Vascular Surgery, vol. 52, no. 4, pp. 1003-1014. https://doi.org/10.1016/j.jvs.2010.05.088
Santora, Rachel J. ; Lie, Mihaela L. ; Grigoryev, Dmitry N. ; Nasir, Omer ; Moore, Frederick A. ; Hassoun, Heitham T. / Therapeutic distant organ effects of regional hypothermia during mesenteric ischemia-reperfusion injury. In: Journal of Vascular Surgery. 2010 ; Vol. 52, No. 4. pp. 1003-1014.
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