Theoretical aspects of single pulse induced modulation of cell-cell interactions favoring iso-electrofusion

Subrata Biswas, Sujoy K. Guha

Research output: Contribution to journalArticle

Abstract

A single pulse-mediated electrofusion of freely suspending widely separated cells could reduce steps in the protocol. Prediction of such electrofusion is still unsolved. In pre-pulse condition, quantitative estimation with three-layered model cell surface reveals electrostatic repulsion (due to negative surface charge) is the major hindrance to membrane-to-membrane contact. On theoretical grounds we predict that designed single high voltage pulsing on widely separated model cell surface can counteract the non-specific repulsive interaction and favor approach of two apposed membranes. But hydrodynamic modulation of pulse exposed cell surface interaction can hamper approach of membranes contact when cells are held at a gap of more than ~450 A. Copyright (C) 1999 Elsevier Science S.A.

Original languageEnglish (US)
Pages (from-to)423-429
Number of pages7
JournalBioelectrochemistry and Bioenergetics
Volume48
Issue number2
DOIs
StatePublished - May 1 1999

Keywords

  • Cell-cell interactions
  • Iso-electrofusion
  • Model cell surface

ASJC Scopus subject areas

  • Biophysics
  • Physical and Theoretical Chemistry
  • Electrochemistry

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